Immunogen
Synthetic peptide from the mid-protein of Human ATG10
Antibody's target
Human ATG10
Antibody's full description
Rabbit Anti-Human ATG10 Polyclonal
100 µg
SPC-669D-ALP
391€
Synthetic peptide from the mid-protein of Human ATG10
Human ATG10
Rabbit Anti-Human ATG10 Polyclonal
Neuroscience, Neurodegeneration, Cancer, Tumor suppressors, Cardiovascular System, Heart, Cancer, Autophagy, Cell Signaling, Metabolism, Metabolism processes
Polyclonal Antibodies
APG 10L Antibody, APG10 like Antibody, Apg 10 Antibody, DKFZP586I0418 Antibody, ATG10 Antibody, ORF Names: Antibody, Ubiquitin-like-conjugating enzyme ATG10 Antibody, APG10, S. cerevisiae, homolog of Antibody, ATG10_HUMAN Antibody, ATG 10 Antibody, APG10-like Antibody, Autophagy related protein 10 Antibody, APG10 Antibody, Autophagy-related protein 10 Antibody, autophagy 10, S. cerevisiae, homolog of Antibody, APG10L Antibody, ATG10 autophagy related 10 homolog Antibody, ATG10 autophagy related 10 homolog (S. cerevisiae) Antibody, FLJ13954 Antibody, PP12616 Antibody, APG10 autophagy 10 like Antibody, pp12616 Antibody
WB
Rabbit
Human
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~25 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-669 was sufficient for detection of ATG10 in 15 µg of human HEK293T cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm
See included datasheet or contact our support service.
Refer to PubMed
25/Oct/2016
NP_001124500.1
83734
Q9H0Y0
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HEK293 cell lysates showing detection of ~25.3 kDa ATG10 protein using Rabbit Anti-ATG10 Polyclonal Antibody (SPC-669). Lane 1: Molecular Weight Ladder (MW). Lane 2: Hek293T cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody (SPC-669) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~25.3 kDa. Rabbit Anti-ATG10 Antibody used in Western blot (WB) on HEK293 cell lysates (SPC-669)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus