Immunogen
Synthetic peptide from the N-terminal of Human WDFY3 (aa. 533-544)
Antibody's target
Human WDFY3
Antibody's full description
Rabbit Anti-Human WDFY3 Polyclonal
100 µg
SPC-666D-ALP
391€
Synthetic peptide from the N-terminal of Human WDFY3 (aa. 533-544)
Human WDFY3
Rabbit Anti-Human WDFY3 Polyclonal
Cancer, Autophagy, Cardiovascular System, Heart
Polyclonal Antibodies
WDFY3 Antibody, Alfy Antibody, WD repeat and FYVE domain-containing protein 3 Antibody, Autophagy-linked FYVE protein Antibody, KIAA0993 Antibody
WB
Rabbit
Human, Rat
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~400 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-666 was sufficient for detection of WDFY3 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Nucleus Membrane, Cytoplasm, Nucleus, Pml Body, Membrane, Peripheral Membrane Protein, Cytoplasmic Side
Ubiquitous. Expressed in osteoclast and their mononuclear precursors (at protein level).
See included datasheet or contact our support service.
Refer to PubMed
29/Aug/2016
NP_055806.2
23001
Q8IZQ1
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 395.2 kDa WDFY3 protein using Rabbit Anti-WDFY3 Polyclonal Antibody (SPC-666). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody (SPC-666) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 395.2 kDa. Rabbit Anti-WDFY3 Antibody used in Western blot (WB) on HeLa and 293Trap cell lysates (SPC-666)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
