Immunogen
Synthetic peptide from the N-terminal of Human PI 3 Kinase Class 3
Antibody's target
Human PI 3 Kinase Class 3
Antibody's full description
Rabbit Anti-Human PI 3 Kinase Class 3 Polyclonal
100 µg
SPC-661D-ALP
391€
Synthetic peptide from the N-terminal of Human PI 3 Kinase Class 3
Human PI 3 Kinase Class 3
Rabbit Anti-Human PI 3 Kinase Class 3 Polyclonal
Cell Signaling, Metabolism, Lipid and lipoprotein Metabolism, Immunology, Innate Immunity, TLR Signalling
Polyclonal Antibodies
PI3 kinase type 3 Antibody, PI 3 kinase type 3 Antibody, hVps34 Antibody, Phosphatidylinositol 3-kinase p100 subunit Antibody, Phosphatidylinositol 3 kinase p100 subunit Antibody, PI3K type 3 Antibody, PI3-kinase type 3 Antibody, EC:2.7.1.137 Antibody, PK3C3_HUMAN Antibody, PtdIns-3-kinase type 3 Antibody, PIK3C3 Antibody, VPS34 Antibody, Pik3c3 Antibody, Phosphoinositide 3 kinase class 3 Antibody, Phosphatidylinositol 3-kinase catalytic subunit type 3 Antibody, PtdIns 3 kinase type 3 Antibody, Phosphoinositide-3-kinase class 3 Antibody, Phosphatidylinositol 3 kinase class 3 Antibody, Vps 34 Antibody, Phosphatidylinositol 3 kinase catalytic subunit type 3 Antibody, MGC61518 Antibody, Vps34 Antibody, PI3 kinase class 3 Antibody
WB, ICC/IF
Rabbit
Mouse, Rat
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~101.5 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-661 was sufficient for detection of PI 3 Kinase Class 3 in 15 µg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome
Ubiquitously expressed, with a highest expression in skeletal muscle.
See included datasheet or contact our support service.
Refer to PubMed
25/Oct/2016
NP_002638.2
5289
Q8NEB9
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (SPC-661). Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (SPC-661) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite. | Western blot analysis of Rat, Mouse Liver cell lysates showing detection of ~101.5 kDa PI 3 Kinase Class 3 protein using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (SPC-661). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Lane 3: Mouse Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (SPC-661) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~101.5 kDa. Rabbit Anti-PI 3 Kinase Class 3 antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on C2C12 Cells (Mouse Myoblast cell line) (SPC-661) | Rabbit Anti-PI 3 Kinase Class 3 antibody used in Western blot (WB) on Liver cell lysates (SPC-661)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
