Immunogen
Synthetic peptide from the C-terminal of Human FIP200 (aa. 1355-1366)
Antibody's target
Human FIP200
Antibody's full description
Rabbit Anti-Human FIP200 Polyclonal
100 µg
SPC-658D-ALP
391€
Synthetic peptide from the C-terminal of Human FIP200 (aa. 1355-1366)
Human FIP200
Rabbit Anti-Human FIP200 Polyclonal
Cell Signaling, Protein Phosphorylation, Tyrosine Kinases, FAK / PYK, Cell Signaling, Metabolism, Cancer, Autophagy
Polyclonal Antibodies
RB1CC1 Antibody, FAK family kinase-interacting protein of 200 kDa Antibody, FIP200 Antibody, KIAA0203 Antibody, RBICC Antibody, RB1-inducible coiled-coil protein 1 Antibody
WB, ICC/IF
Rabbit
Human, Rat
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~150 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-658 was sufficient for detection of FIP200 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Nucleus, Cytoplasm, Cytoplasm, Cytosol, Preautophagosomal Structure
Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells.
See included datasheet or contact our support service.
Refer to PubMed
29/Aug/2016
NP_001077086.1
9821
Q8TDY2
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-FIP200 Polyclonal Antibody (SPC-658). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-FIP200 Polyclonal Antibody (SPC-658) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus, Cytoplasm, Cytoplasm, Cytosol, Preautophagosomal Structure. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FIP200 Antibody (D) Composite. | Western blot analysis of Rat Brain cell lysates showing detection of 183 kDa FIP200 protein using Rabbit Anti-FIP200 Polyclonal Antibody (SPC-658). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FIP200 Polyclonal Antibody (SPC-658) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 183 kDa. Rabbit Anti-FIP200 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-658) | Rabbit Anti-FIP200 Antibody used in Western blot (WB) on Brain cell lysates (SPC-658)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
