Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Size

100 µg

Catalog No

SPC-657D-ALP

Price

391€

Immunogen

Synthetic peptide from the mid-protein range of human ATG13

Antibody's target

Human ATG13

Antibody's full description

Rabbit Anti-Human ATG13 Polyclonal

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Primary research fields

Cancer, Autophagy

Antibody's category

Polyclonal Antibodies

Antibody's other name

ATG13_Human Antibody, Autophagy related protein 13 Antibody, KIAA0652 Antibody

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Verified applications

WB, ICC/IF

Raised in

Rabbit

Antibody's reactivity

Human, Mouse

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Antibody's recommended dilutions for use

WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody's purified from

Peptide Affinity Purified

Recommended buffer for storage

PBS, 50% glycerol, 0.09% sodium azide

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Antibody's concentration

1 mg/ml

Antibody's specificity

Detects ~56-63kDa.

Storage recommendations

-20°C

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Shipping recommendations

Blue Ice or 4°C

Certificate of analysis

A 1:1000 dilution of SPC-657 was sufficient for detection of ATG13 on HEK293 lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody in cell

Cytoplasm, Cytosol, Preautophagosomal structure

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Tissue specificity

See included datasheet or contact our support service.

Scientific context

ATG13 is an autophagy factor required for phagosome formation. It is a target of the TOR kinase signaling pathway that regulates autophagy through phosphorylation of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (1-3).

Bibliography

1. Mercer C.A., Kaliappan A., Dennis P.B. (2009) Autophagy. 5(5): 649-662. 2. Hosokawa N., et al. (2009) Mol Biol Cell. 20(7): 1981-1991. 3. Chan E.Y., Longatti A., McKnight N.C., Tooze S.A. (2009) Mol Cell Biol. 29(1): 157-171.

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Released date

1/Oct/2015

NCBI number

NP_001136145.1

Gene number

9776

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Protein number

O75143

PubMed number

Refer to PubMed

Tested applications

To be tested

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Tested reactivity

To be tested

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure link

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Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Representative figure legend

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG13 Polyclonal Antibody (SPC-657). Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG13 Polyclonal Antibody (SPC-657) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG13 Antibody (D) Composite. | Western blot analysis of Human HEK293 showing detection of ~56.6kDa ATG13 protein using Rabbit Anti-ATG13 Polyclonal Antibody (SPC-657). Lane 1: MW Ladder. Lane 2: Human HEK293 (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG13 Polyclonal Antibody (SPC-657) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~56.6kDa. Rabbit Anti-ATG13 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Myoblast cell line (C2C12) (SPC-657) | Rabbit Anti-ATG13 Antibody used in Western blot (WB) on HEK293 (SPC-657)

Warning information

Non-hazardous

Country of production

Canada

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Total weight (kg)

1.4

Net weight (g)

0.1

Stock availabilit

In Stock

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus

Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase
Rabbit Anti-Human ATG13 Polyclonal: Alkaline Phosphatase