Immunogen
Synthetic peptide from the C-terminal of human ATG2A
Antibody's target
Human ATG2A
Antibody's full description
Rabbit Anti-Human ATG2A Polyclonal
100 µg
SPC-652D-ALP
391€
Synthetic peptide from the C-terminal of human ATG2A
Human ATG2A
Rabbit Anti-Human ATG2A Polyclonal
Neuroscience, Cancer, Autophagy, Cardiovascular System, Heart
Polyclonal Antibodies
ATG2 autophagy related homolog A Antibody, ATG2a_HUMAN Antibody, BC023754 Antibody, KIAA0404 Antibody, 1810013C15Rik Antibody
WB, ICC/IF
Rabbit
Human, Mouse
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Predicted molecular weights at ~213kDa. Other observed bands around ~90-100kDa to be expected.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-652 was sufficient for detection of ATG2A on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Preautophagosomal Structure Membrane, Peripheral membrane protein
See included datasheet or contact our support service.
ATG2A belongs to the ATG2 family. It is required for both autophagosome formation and regulation of lipid droplet morphology and dispersion (1).
1. Velikkakath A.K., Nishimura T., Oita E., Ishihara N., Mizushima N. (2012) Mol Biol Cell. 23(5): 896-909.
1/Oct/2015
NP_055919.2
23130
Q2TAZ0
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG2A Antibody (D) Composite. | Western blot analysis of Mouse Brain showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa. | Western blot analysis of Human HeLa cell lysates showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa. Rabbit Anti-ATG2A Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-652) | Rabbit Anti-ATG2A Antibody used in Western blot (WB) on Brain (SPC-652) | Rabbit Anti-ATG2A Antibody used in Western blot (WB) on HeLa cell lysates (SPC-652)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
