Immunogen
Synthetic peptide from the N-terminal of Human ATG9B (aa. 110-121)
Antibody's target
Human ATG9B
Antibody's full description
Rabbit Anti-Human ATG9B Polyclonal
100 µg
SPC-646D-ALP
391€
Synthetic peptide from the N-terminal of Human ATG9B (aa. 110-121)
Human ATG9B
Rabbit Anti-Human ATG9B Polyclonal
Cardiovascular System, Heart, Cancer, Autophagy, Cell Signaling, Metabolism, Mitochondrial Metabolism
Polyclonal Antibodies
APG9-like 2 Antibody, APG9L2 Antibody, Nitric oxide synthase 3-overlapping antisense gene protein Antibody, Protein sONE Antibody, NOS3AS Antibody, ATG9B Antibody, Autophagy-related protein 9B Antibody,
WB, ICC/IF
Rabbit
Human, Mouse
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~100 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-646 was sufficient for detection of ATG9B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasmic Vesicle, Autophagosome Membrane, Multi-Pass Membrane Protein
Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial.
ATG9B is involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Highly expressed in the placenta and the pituitary gland.
29/Aug/2016
NP_001303985.1
285973
Q674R7
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG9B Antibody (D) Composite. | Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa. Rabbit Anti-ATG9B Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-646) | Rabbit Anti-ATG9B Antibody used in Western blot (WB) on Brain cell lysates (SPC-646)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
