Immunogen
Synthetic peptide from the N-terminal of Human ATG4D (aa. 98-108)
Antibody's target
Human ATG4D
Antibody's full description
Rabbit Anti-Human ATG4D Polyclonal
100 µg
SPC-635D-ALP
391€
Synthetic peptide from the N-terminal of Human ATG4D (aa. 98-108)
Human ATG4D
Rabbit Anti-Human ATG4D Polyclonal
Cardiovascular System, Heart, Cancer, Autophagy
Polyclonal Antibodies
Autophagin-4 Antibody, APG4D Antibody, ATG4D Antibody, Cysteine protease ATG4D Antibody, AUTL4 Antibody, Autophagy-related cysteine endopeptidase 4 Antibody, EC:3.4.22.- Antibody, AUT-like 4 cysteine endopeptidase Antibody, Autophagy-related protein 4 homolog D Antibody,
WB
Rabbit
Human
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~53 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-635 was sufficient for detection of ATG4D in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm
Mainly expressed in skeletal muscle and, to a lower extent, in testis.
ATG4D, Auotphagy related 4D, cysteine peptidases, belong to the autophagy-related protein 4 family of C54 endopeptidases. Members of this family play a role in the biogenesis of autophagosomes (1). Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (2).
1. "Entrez Gene: Autophagy related 4D, cysteine peptidase". 2. Li M., et al. (2011) J Biol Chem. 286: 7327-7338.
29/Aug/2016
NP_001268433.1
84971
Q86TL0
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa cell lysates showing detection of 52.9 kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 52.9 kDa. Other Band(s): ~45 and 48 kDa. Rabbit Anti-ATG4D Antibody used in Western blot (WB) on HeLa cell lysates (SPC-635)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
