Immunogen
Synthetic peptide from the C-terminal of Human ATG4C (aa. 296-306)
Antibody's target
Human ATG4C
Antibody's full description
Rabbit Anti-Human ATG4C Polyclonal
100 µg
SPC-633D-ALP
391€
Synthetic peptide from the C-terminal of Human ATG4C (aa. 296-306)
Human ATG4C
Rabbit Anti-Human ATG4C Polyclonal
Cancer, Cardiovascular System, Autophagy
Polyclonal Antibodies
AUT like 1cysteine endopeptidase (S. cerevisiae) Antibody, APG4 autophagy 4 homolog C Antibody, Cysteine protease ATG4C Antibody, APG4-C Antibody, Autophagy-related cysteine endopeptidase 3 Antibody, EC:3.4.22.- Antibody, ATG 4C Antibody, AUT like 1, cysteine endopeptidase Antibody, EC 3.4.22 Antibody, APG4C Antibody, Autophagin-3 Antibody, OTTHUMP00000010715 Antibody, AUTL3 Antibody, APG4 autophagy 4 homolog C (S. cerevisiae) Antibody, Autophagy related cysteine endopeptidase 3 Antibody, ATG4C Antibody, Autophagin 3 Antibody, ATG4 autophagy related 4 homolog C Antibody, AUT like 3 cysteine endopeptidase Antibody, AUTL1 Antibody, ATG4C_HUMAN Antibody, AUT (S. cerevisiae) like 1, cysteine endopeptidase Antibody, Autophagy-related protein 4 homolog C Antibody, Autophagy related protein 4 homolog C Antibody, ATG4 autophagy related 4 homolog C (S. cerevisiae) Antibody, Autophagy related 4C cysteine peptidase Antibody, Atg4c Antibody, APG4 C Antibody, FLJ14867 Antibody, AUT-like 3 cysteine endopeptidase Antibody,
WB
Rabbit
Human
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~50 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-633 was sufficient for detection of ATG4C in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm
Highly expressed in skeletal muscle, heart, liver and testis.
See included datasheet or contact our support service.
Refer to PubMed
29/Aug/2016
NP_116241.2
84938
Q96DT6
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HEK293T cell lysates showing detection of 53 kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody (SPC-633). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HEK293T cell lysates. Load: 15 µg . Block: 2% GE Ashermsham in 1X TBST. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody (SPC-633) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 minutes in RT. Predicted/Observed Size: 53 kDa. Rabbit Anti-ATG4C Antibody used in Western blot (WB) on HEK293T cell lysates (SPC-633)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
