Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Size

100 µg

Catalog No

SPC-630D-ALP

Price

391€

Immunogen

Synthetic peptide from the N-terminal to mid of Human ATG4B (aa. 94-106)

Antibody's target

Human ATG4B

Antibody's full description

Rabbit Anti-Human ATG4B Polyclonal

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Primary research fields

Cancer, Autophagy, Cardiovascular System, Heart, Cell Signaling

Antibody's category

Polyclonal Antibodies

Antibody's other name

MGC1353 Antibody, Autophagy-related cysteine endopeptidase 1 Antibody, ATG4B Antibody, hAPG4B Antibody, AUT-like 1 cysteine endopeptidase Antibody, Cysteine protease ATG4B Antibody, Autophagy-related protein 4 homolog B Antibody, Autophagin-1 Antibody, EC:3.4.22.- Antibody, ATG4B_HUMAN Antibody, APG4B Antibody, APG4 autophagy 4 homolog B Antibody, AUTL1 Antibody, KIAA0943 Antibody, Autophagy related cysteine endopeptidase 1 Antibody, Autophagin 1 Antibody, ATG4 autophagy related 4 homolog B (S. cerevisiae) Antibody, AUT like 1 cysteine endopeptidase Antibody, Autophagy related protein 4 homolog B Antibody,

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Verified applications

WB, ICC/IF

Raised in

Rabbit

Antibody's reactivity

Human, Mouse

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Antibody's recommended dilutions for use

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody's purified from

Peptide Affinity Purified

Recommended buffer for storage

PBS, 50% glycerol, 0.09% sodium azide

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Antibody's concentration

1 mg/ml

Antibody's specificity

Detects ~44 kDa.

Storage recommendations

-20°C

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Shipping recommendations

Blue Ice or 4°C

Certificate of analysis

A 1:1000 dilution of SPC-630 was sufficient for detection of ATG4B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody in cell

Cytoplasm

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Tissue specificity

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

Scientific context

Autophagy related 4B, cysteine peptidase (ATG4B or APG4B) is a C-54 cysteine protease that also belongs to the family of autophagin poteins. Specifically it cleaves LC3, and mutations can be associated with strong inhibition of autophagosome formation.

Bibliography

1. Kabeya Y., et al. (2004) J Cell Sci. 117: 2805-2812. 2. Tanida I., et al. (2004) J Biol Chem. 279: 36268-36276. 3. Hemelaar J., et al. (2003) J Biol Chem. 278: 51841-51850.

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Released date

29/Aug/2016

NCBI number

NP_037457.3

Gene number

23192

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Protein number

Q9Y4P1

PubMed number

Refer to PubMed

Tested applications

To be tested

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Tested reactivity

To be tested

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure link

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Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Representative figure legend

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4B Polyclonal Antibody (SPC-630). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody (SPC-630) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4B Antibody (D) Composite. | Western blot analysis of Mouse Brain cell lysates showing detection of ATG4B protein using Rabbit Anti-ATG4B Polyclonal Antibody (SPC-630). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody (SPC-630) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Other Band(s): Band appears at ~40 kDa. Rabbit Anti-ATG4B Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-630) | Rabbit Anti-ATG4B Antibody used in Western blot (WB) on Brain cell lysates (SPC-630)

Warning information

Non-hazardous

Country of production

Canada

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Total weight (kg)

1.4

Net weight (g)

0.1

Stock availabilit

In Stock

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus

Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase
Rabbit Anti-Human ATG4B Polyclonal: Alkaline Phosphatase