Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Size

100 µg

Catalog No

SPC-629D-ALP

Price

391€

Immunogen

Synthetic peptide from the N-terminal of Human ATG4A (aa. 58-70)

Antibody's target

Human ATG4A

Antibody's full description

Rabbit Anti-Human ATG4A Polyclonal

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Primary research fields

Cancer, Autophagy, Cardiovascular System, Heart, Cell Signaling

Antibody's category

Polyclonal Antibodies

Antibody's other name

ATG4 autophagy related 4 homolog A (S. cerevisiae) Antibody, MGC107179 Antibody, AV169859 Antibody, Autophagy-related cysteine endopeptidase 2 Antibody, hAPG4A Antibody, EC:3.4.22.- Antibody, ATG4A Antibody, Autophagin 2 Antibody, Autophagy related protein 4 homolog A Antibody, Cysteine protease ATG4A Antibody, AUTL2 Antibody, Autl2 Antibody, Autophagy-related protein 4 homolog A Antibody, Atg4al Antibody, Autophagin-2 Antibody, Autophagy related 4A cysteine peptidase Antibody, AUT like 2 cysteine endopeptidase Antibody, Autophagy related cysteine endopeptidase 2 Antibody, Apg4a Antibody, AUT-like 2 cysteine endopeptidase Antibody, APG4A Antibody, AI627006 Antibody, ATG4A_HUMAN Antibody,

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Verified applications

WB, ICC/IF

Raised in

Rabbit

Antibody's reactivity

Human

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Antibody's recommended dilutions for use

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody's purified from

Peptide Affinity Purified

Recommended buffer for storage

PBS, 50% glycerol, 0.09% sodium azide

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Antibody's concentration

1 mg/ml

Antibody's specificity

Detects ~48 kDa.

Storage recommendations

-20°C

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Shipping recommendations

Blue Ice or 4°C

Certificate of analysis

A 1:1000 dilution of SPC-629 was sufficient for detection of ATG4A in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody in cell

Cytoplasm

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Tissue specificity

Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.

Scientific context

See included datasheet or contact our support service.

Bibliography

Refer to PubMed

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Released date

29/Aug/2016

NCBI number

NP_001308216.1

Gene number

115201

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Protein number

Q8WYN0

PubMed number

Refer to PubMed

Tested applications

To be tested

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Tested reactivity

To be tested

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure link

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Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Representative figure legend

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody (SPC-629). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (SPC-629) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite. | Western blot analysis of Human HeLa cell lysates showing detection of 45.3 kda ATG4A protein using Rabbit Anti-ATG4A Polyclonal Antibody (SPC-629). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody (SPC-629) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 45.3 kda. Rabbit Anti-ATG4A Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-629) | Rabbit Anti-ATG4A Antibody used in Western blot (WB) on HeLa cell lysates (SPC-629)

Warning information

Non-hazardous

Country of production

Canada

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Total weight (kg)

1.4

Net weight (g)

0.1

Stock availabilit

In Stock

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus

Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase
Rabbit Anti-Human ATG4A Polyclonal: Alkaline Phosphatase