Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Size

100 µg

Catalog No

SPC-624D-ALP

Price

391€

Immunogen

Synthetic peptide from the C-terminal of Human GABARAPL2

Antibody's target

Human GABARAPL2

Antibody's full description

Rabbit Anti-Human GABARAPL2 Polyclonal

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Primary research fields

Cell Signaling, Protein Trafficking, Vesicle Transport, Cancer, Autophagy, Cell Signaling, Metabolism, Metabolism processes

Antibody's category

Polyclonal Antibodies

Antibody's other name

ATG8 Antibody, FLC3A Antibody, General protein transport factor p16 Antibody, GEF-2 Antibody, GABARAPL2 Antibody, GBRL2_HUMAN Antibody, GEF2 Antibody, GABA(A) receptor-associated protein-like 2 Antibody, MAP1 light chain 3-related protein Antibody, Golgi-associated ATPase enhancer of 16 kDa Antibody, Gabarapl2 Antibody, GATE-16 Antibody, Gamma-aminobutyric acid receptor-associated protein-like 2 Antibody, Ganglioside expression factor 2 Antibody, ATG8C Antibody, GATE16 Antibody

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Verified applications

WB

Raised in

Rabbit

Antibody's reactivity

Human, Rat

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Antibody's recommended dilutions for use

WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody's purified from

Peptide Affinity Purified

Recommended buffer for storage

PBS, 50% glycerol, 0.09% sodium azide

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Antibody's concentration

1 mg/ml

Antibody's specificity

Detects ~14 kDa.

Storage recommendations

-20°C

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Shipping recommendations

Blue Ice or 4°C

Certificate of analysis

A 1:1000 dilution of SPC-624 was sufficient for detection of GABARAPL2 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody in cell

Golgi Apparatus, Cytoplasmic Vesicle, Autophagosome

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Tissue specificity

Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.

Scientific context

See included datasheet or contact our support service.

Bibliography

Refer to PubMed

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Released date

25/Oct/2016

NCBI number

NP_009216.1

Gene number

11345

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Protein number

P60520

PubMed number

Refer to PubMed

Tested applications

To be tested

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Tested reactivity

To be tested

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure legend

Western blot analysis of Rat Liver cell lysates showing detection of ~14 kDa GABARAPL2 protein using Rabbit Anti-GABARAPL2 Polyclonal Antibody (SPC-624). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL2 Polyclonal Antibody (SPC-624) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~14 kDa. Rabbit Anti-GABARAPL2 Antibody used in Western blot (WB) on Liver cell lysates (SPC-624)

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Warning information

Non-hazardous

Country of production

Canada

Total weight (kg)

1.4

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Net weight (g)

0.1

Stock availabilit

In Stock

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Group

Polyclonals and antibodies

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus

Rabbit Anti-Human GABARAPL2 Polyclonal: Alkaline Phosphatase