Immunogen
Synthetic peptide from the N-terminal of Human GABARAPL1 (aa. 5-16)
Antibody's target
Human GABARAPL1
Antibody's full description
Rabbit Anti-Human GABARAPL1 Polyclonal
100 µg
SPC-622D-ALP
391€
Synthetic peptide from the N-terminal of Human GABARAPL1 (aa. 5-16)
Human GABARAPL1
Rabbit Anti-Human GABARAPL1 Polyclonal
Neuroscience, Neurotransmitter Receptors, GABA Receptors
Polyclonal Antibodies
APG8 like Antibody, GEC-1 Antibody, ATG8 Antibody, Glandular epithelial cell protein 1 Antibody, ATG8B Antibody, GEC1 Antibody, GBRL1_HUMAN Antibody, Gamma-aminobutyric acid receptor-associated protein-like 1 Antibody, GABARAPL1 a Antibody, ATG8L Antibody, APG8L Antibody, GABA(A) receptor-associated protein-like 1 Antibody, Early estrogen regulated protein Antibody, Early estrogen-regulated protein Antibody, GABA(A) receptor associated protein like 1 Antibody, Gamma aminobutyric acid receptor associated protein like 1 Antibody, GEC 1 Antibody, GABARAPL1 Antibody, GABA Antibody,
WB, ICC/IF
Rabbit
Human, Mouse
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~18 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-622 was sufficient for detection of GABARAPL1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Cytoskeleton, Cytoplasmic Vesicle Membrane, Lipid-Anchor, Endoplasmic Reticulum, Golgi Apparatus, Cytoplasmic Vesicle, Autophagosome
Ubiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
See included datasheet or contact our support service.
Refer to PubMed
29/Aug/2016
NP_113600.1
23710
Q9H0R8
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome, Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL1 Antibody (D) Composite. | Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 14 kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa. Rabbit Anti-GABARAPL1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-622) | Rabbit Anti-GABARAPL1 Antibody used in Western blot (WB) on HeLa and 293Trap cell lysates (SPC-622)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
