Immunogen
Synthetic peptide from the N-terminal of Human LC3A (aa. 1-12)
Antibody's target
Human LC3A
Antibody's full description
Rabbit Anti-Human LC3A Polyclonal
100 µg
SPC-613D-ALP
391€
Synthetic peptide from the N-terminal of Human LC3A (aa. 1-12)
Human LC3A
Rabbit Anti-Human LC3A Polyclonal
Cancer, Autophagy, Cell Signaling, Neuroscience, Cardiovascular System, Heart
Polyclonal Antibodies
MAP1 light chain 3 like protein 1 Antibody, Map1lc3a Antibody, MAP1BLC3 Antibody, Autophagy-related ubiquitin-like modifier LC3 A Antibody, MAP1LC3A Antibody, LC3A Antibody, Microtubule-associated proteins 1A and 1B, light chain 3 Antibody, MAP1ALC3 Antibody, MAP1A/1B light chain 3 A Antibody, MAP1A/MAP1B light chain 3 A Antibody, Microtubule-associated protein 1 light chain 3 alpha Antibody, LC3 Antibody, Microtubule-associated proteins 1A/1B light chain 3A Antibody, Autophagy-related protein LC3 A Antibody, MAP1A/MAP1B LC3 A Antibody, ATG8E Antibody, MLP3A_HUMAN Antibody, MAP1 light chain 3-like protein 1 Antibody, Microtubule associated proteins 1A/1B light chain 3 Antibody,
WB, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~14 kDa. Band at 50 kDa is LC3A complex.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-613 was sufficient for detection of LC3A in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Cytoskeleton, Endomembrane System, Lipid-Anchor, Cytoplasmic Vesicle, Autophagosome Membrane, Lipid-Anchor, Cytoplasmic Vesicle, Autophagosome
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.
1. Tanida I., Ueno T., & Kominami E. (2008) Methods Mol Biol. 445:77-88. 2. He H., et al. (2003) J Biol Chem. 278(31): 29278-87.
29/Aug/2016
NP_115903.1
84557
Q9H492
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-LC3A Polyclonal Antibody (SPC-613). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-LC3A Polyclonal Antibody (SPC-613) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Autophagosome, Cytoplasmic Vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LC3A Antibody (D) Composite. | Western blot analysis of Rat Liver cell lysates showing detection of 14 kDa LC3A protein using Rabbit Anti-LC3A Polyclonal Antibody (SPC-613). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-LC3A Polyclonal Antibody (SPC-613) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa. Rabbit Anti-LC3A Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-613) | Rabbit Anti-LC3A Antibody used in Western blot (WB) on Liver cell lysates (SPC-613)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
