Immunogen
Synthetic peptide from the C-terminal of human ATG12
Antibody's target
Human ATG12
Antibody's full description
Rabbit Anti-Human ATG12 Polyclonal
100 µg
SPC-608D-ALP
391€
Synthetic peptide from the C-terminal of human ATG12
Human ATG12
Rabbit Anti-Human ATG12 Polyclonal
Cancer, Autophagy, Cell Signaling, Epigenetics, Cardiovascular System, Heart
Polyclonal Antibodies
APG12-like Antibody, APG12 Antibody, ATG12_Human Antibody, HAPG12 Antibody, FBR93 Antibody
WB, ICC/IF
Rabbit
Human
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Predicted molecular weight at ~15.1kDa. Observed molecular weights at ~48-55kDa based on ATG12-ATG5 heterodimer.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-608 was sufficient for detection of ATG12 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm
See included datasheet or contact our support service.
ATG12 (autophagy-related protein 12), also known as APG12, is a 140 amino acid protein that is ubiquitously expressed and belongs to the ATG12 family of proteins. ATG12 is a homolog of the yeast protein APG12 that participates in autophagy. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling. In yeast, autophagy requires a protein conjugation system consisting of APG12 covalently bound at the carboxy terminal glycine to lysine 149 of APG5. Similarly in humans, ATG12 is essential for autophagy and localizes to the cytoplasm where it is covalently bound to APG5, a conjugation reaction that requires APG7, ATG10 and ATP (1-3). The ATG12-APG5 conjugate functions as an important regulator of the autophagic process and is required for the change in membrane morphology and development of autophagosomes. Due to alternative splicing events, two ATG12 isoforms exist.
1. Mizushima N., Sugita H., Yoshimori T., Ohsumi Y. (1999) J Biol Chem. 273(51): 33889-33892. 2. Mizushuma N., et al. (1998) Nature. 395: 395-398. 3. Suzuki K., et al. (2001) EMBO J. 20: 5971-5981.
1/Oct/2015
NP_001264712.1
9140
O94817
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG12 Polyclonal Antibody (SPC-608). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody (SPC-608) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG12 Antibody (D) Composite. | Western blot analysis of Human HeLa cell lysates showing detection of ~15kDa ATG12 protein using Rabbit Anti-ATG12 Polyclonal Antibody (SPC-608). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody (SPC-608) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~15kDa. Other Band(s): ATG12-ATG5 Complex. Rabbit Anti-ATG12 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cervical Cancer cell line (HeLa) (SPC-608) | Rabbit Anti-ATG12 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-608)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
