Immunogen
Synthetic peptide from the mid-protein of human UVRAG
Antibody's target
Human UVRAG
Antibody's full description
Rabbit Anti-Human UVRAG Polyclonal
100 µg
SPC-606D-ALP
391€
Synthetic peptide from the mid-protein of human UVRAG
Human UVRAG
Rabbit Anti-Human UVRAG Polyclonal
Cancer, Apoptosis, Autophagy, Cell Signaling, Epigenetics, Cardiovascular System, Heart
Polyclonal Antibodies
DHTX Antibody, p63 Antibody, UV radiation resistance associated Antibody, UVRAG_HUMAN Antibody
WB, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Predicted molecular weight at ~78.1kDa. Observed molecular weights in the 75-90kDa range.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-606 was sufficient for detection of UVRAG on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Endosome, Lysosome, Endoplasmic reticulum, Nucleus, Chromosome, Centromere
See included datasheet or contact our support service.
UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (1). Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (2). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (3-4). It is highly expressed in the brain, lung, kidney and liver.
1. Liang, C. et al. (2008) Nat Cell Biol. 10: 776-87. 2. Liang, C. et al. (2008) Nat Cell Biol. 10: 776-87. 3. Ionov, Y. et al. (2004) Oncogene. 23: 639-45. 4. Kim, M.S. et al. (2008) Hum Pathol. 39: 1059-63.
1/Oct/2015
NP_003360.2
7405
Q9P2Y5
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-UVRAG Polyclonal Antibody (SPC-606). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (SPC-606) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Late Endosome, Lysosome, Early Endosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) UVRAG Antibody (D) Composite. | Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~100kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody (SPC-606). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (SPC-606) at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~100kDa. | Western blot analysis of Rat Liver showing detection of ~100kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody (SPC-606). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (SPC-606) at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~100kDa. Rabbit Anti-UVRAG Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-606) | Rabbit Anti-UVRAG Antibody used in Western blot (WB) on HeLa and 293T cell lysates (SPC-606) | Rabbit Anti-UVRAG Antibody used in Western blot (WB) on Liver (SPC-606)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
