Immunogen
Synthetic peptide from the C-terminal of Human Beclin 2 (aa. 338-349)
Antibody's target
Human Beclin 2
Antibody's full description
Rabbit Anti-Human Beclin 2 Polyclonal
100 µg
SPC-603D-ALP
391€
Synthetic peptide from the C-terminal of Human Beclin 2 (aa. 338-349)
Human Beclin 2
Rabbit Anti-Human Beclin 2 Polyclonal
Cell Signaling, Epigenetics and Nuclear Signalling, Transcription, Cancer, Tumor suppressors, Cancer, Autophagy, Cancer, Cancer Metabolism
Polyclonal Antibodies
BECN1P1 Antibody, Beclin-1 autophagy-related pseudogene 1 Antibody, BECN2 Antibody, Beclin-2 Antibody, BECN1L1 Antibody, Beclin-1-like protein 1 Antibody,
WB, ICC/IF
Rabbit
Human, Rat
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~48 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-603 was sufficient for detection of Beclin 2 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm
Present in fetal and adult brain (at protein level).
Beclin 2 is a novel coiled-coil protein related to the autophagic Beclin 1 protein. It is thought to interact with Bcl-2, an anti-apoptotic protein, and is believed to function in autophagy. It has alson been found to mediate G protein-coupled receptro (GPCR) degradation (1).
1. He C., et al. (2013) Cell. 154(5): 1085-1099.
29/Aug/2016
NP_001277622.1
441925
A8MW95
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-603). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-603) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 2 Antibody (D) Composite. | Western blot analysis of Rat Liver cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-603). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-603) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa. Rabbit Anti-Beclin 2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-603) | Rabbit Anti-Beclin 2 Antibody used in Western blot (WB) on Liver cell lysates (SPC-603)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
