Immunogen
Synthetic peptide from the mid-protein of human Beclin 1
Antibody's target
Human Beclin 1
Antibody's full description
Rabbit Anti-Human Beclin 1 Polyclonal
100 µg
SPC-600D-ALP
391€
Synthetic peptide from the mid-protein of human Beclin 1
Human Beclin 1
Rabbit Anti-Human Beclin 1 Polyclonal
Cell Signaling, Epigenetics and Nuclear Signalling, Transcription, Cancer, Tumor suppressors, Cancer, Autophagy, Cancer, Cancer Metabolism
Polyclonal Antibodies
APG6 Antibody, BCL-2 interacting protein beclin Antibody, Beclin 1 autophagy related Antibody, BECN1 Antibody, BECN1_HUMAN Antibody, GT197 Antibody, VPS30 Antibody
WB, ICC/IF
Rabbit
Human, Rat
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Predicted molecular weight at ~51kDa. Observed a secondary band on some lysates at ~30kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-600 was sufficient for detection of Beclin1 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Golgi apparatus, Trans-Golgi network membrane| Endosome, Endoplasmic reticulum membrane, Mitochondrion, Mitochondrion membrane, Cytoplasmic vesicle, Autophagosome
See included datasheet or contact our support service.
Beclin 1 is the mammalian ortholog of the yeast autophagy -related gene Atg6. It regulates autophagy and has an important role in development, tumorigenesis and neurodegeneration (1). Beclin 1 is localized within cytoplasmic structures including the mitochondria, yet overexpression reveals some nuclear staining (2). Researchers have found that schizonphrenia is associated with low levels of Beclin-1 in the hippocampus (3). It may also protect against infection by a neuorvirulent strain of Sindbis virus (4).
1. Zhong Y., et al. (2009) Nat Cell Biol. 11(4): 468-476. 2. Liang X.H., et al. (2001) Cancer Res. 61: 3443-3449. 3. Merenlender-Wagner A., et al. (2013) Mol. Psychiatry 20:126-132. 4. Liang X.H., et al. (1998) J Virol. 72: 8586-8596.
1/Oct/2015
NP_001300927.1
8678
Q14457
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 1 Antibody (D) Composite. | Western blot analysis of Human HeLa cell lysates showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa. | Western blot analysis of Rat Liver showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa. Rabbit Anti-Beclin 1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-600) | Rabbit Anti-Beclin 1 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-600) | Rabbit Anti-Beclin 1 Antibody used in Western blot (WB) on Liver (SPC-600)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
