Immunogen
Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4
Antibody's target
Rat Aquaporin 4
Antibody's full description
Rabbit Anti-Rat Aquaporin 4 Polyclonal
100 µg
SPC-505D-ALP
390€
Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4
Rat Aquaporin 4
Rabbit Anti-Rat Aquaporin 4 Polyclonal
Neuroscience, Ion Channels, Aquaporins
Polyclonal Antibodies
AQP 4 Antibody, AQP-4 Antibody, AQP4 Antibody, AQP4_HUMAN Antibody, Aquaporin type 4 Antibody, Aquaporin-4 Antibody, Aquaporin4 Antibody, HMIWC 2 Antibody, HMIWC2 Antibody, Mercurial insensitive water channel Antibody, Mercurial-insensitive water channel Antibody, MGC22454 Antibody, MIWC Antibody, WCH 4 Antibody, WCH4 Antibody
WB, IHC, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~35kDa. May detect larger glycosylated bands ~50kDa.
-20°C
Blue Ice or 4°C
0.5 µg/ml of SPC-505 was sufficient for detection of aquaporin 4 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Membrane
See included datasheet or contact our support service.
Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 4 is constitutively expressed in the basolateral cell membrane of principal collecting duct cells in the kidney and provide a pathway for water to exit these cells (3). It is also expressed in astrocytes and is up regulated by direct insult to the CNS (4). Aquaporin 4 has been proposed as the primary autoimmune target in neuromyelitis optica ( 5, 6).
1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. Agre P., Nielsen S. (1996) Nephrologie. 17(7): 409-415. 4. Nagelhus E.A., Mathiisen T.M., Ottersen O.P. (2004) Neuroscience. 129(4): 905-913. 5. Lennon V.A., Kryzer T.J., Pittock S.J., Verkman A.S., Hinson S.R. (2005) J Exp Med. 202(4): 473-477. 6. Bradl M., Lassmann D.H. (2008) Int. MS Journal/MS Forum. 15(3): 75-78.
6/Dec/2011
NP_036957.1
25293
P47863
27413198
To be tested
Immunohistochemistry analysis using Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green). | Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 4 protein using Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505). Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505) at 1:2000. Rabbit Anti-Aquaporin 4 Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-505) | Rabbit Anti-Aquaporin 4 Antibody used in Western blot (WB) on Rat kidney inner medullary homogenates (SPC-505)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Oryctolagus cuniculus,Rattus norvegicus
