Immunogen
Produced against the C-terminal tail (amino acids 629-650) of rat gamma ENaC (antibody designation L550)
Antibody's target
Rat ENaC
Antibody's full description
Rabbit Anti-Rat ENaC gamma Polyclonal
100 µg
SPC-405D-ALP
400€
Produced against the C-terminal tail (amino acids 629-650) of rat gamma ENaC (antibody designation L550)
Rat ENaC
Rabbit Anti-Rat ENaC gamma Polyclonal
Neuroscience, Ion Channels, Sodium Channels, Epithelial Sodium Channels (ENaC)
Polyclonal Antibodies
SCNN1G Antibody, Epithelial Sodium Channel gamma Antibody, Amiloride sensitive epithelial sodium channel gamma subunit Antibody, Amiloride sensitive sodium channel subunit gamma Antibody, Amiloride-sensitive sodium channel subunit gamma Antibody, BESC3 Antibody, ENaC gamma subunit Antibody, ENaCG Antibody, ENaCgamma Antibody, Epithelial Na(+) channel subunit gamma Antibody, Epithelial Na+ channel subunit gamma Antibody, Gamma ENaC Antibody, Gamma NaCH Antibody, Gamma-ENaC Antibody, Gamma-NaCH Antibody, Nonvoltage gated sodium channel 1 subunit gamma Antibody, Nonvoltage-gated sodium channel 1 subunit gamma Antibody, PHA 1 Antibody, PHA1 Antibody, SCNEG Antibody, SCNN 1G Antibody, SCNN1G Antibody, SCNNG_HUMAN Antibody, Sodium channel non voltage gated 1 gamma subunit Antibody, Sodium channel nonvoltage gated 1 gamma Antibody
WB, IHC, ICC/IF
Rabbit
Rat, Mouse, African clawed frog (Xenopus laevis), Hamster, Human
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~83kDa.
-20°C
Blue Ice or 4°C
1 µg/ml of SPC-405 was sufficient for detection of gamma-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Apical Cell Membrane
See included datasheet or contact our support service.
The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
11/Jun/2010
NP_058742
24768
P37091
27895120|27443990|27440776|27076431|27068441|27053687|26963391|26892518|26418861|26207612|25209858|25225551|24707975|25164814|25122048|24808538|24429824|24145067|23558339|23264685|22814399|23029554|22847418|22549242|21536685|21536685
Western Blot|Western Blot|Western Blot|Immunohistochemistry|Western Blot|Western Blot|Western Blot|Western Blot|Western Blot|Western Blot||Western Blot|Immunohistochemistry|Western Blot|Western Blot|Western Blot|Western Blot|Immunohistochemistry|Immunohistochemistry|Western Blot|Western Blot|Western Blot|Western Blot|Western Blot|Western Blot|Western Blot
Mouse|Rat|Rat|Mouse|Mouse|Mouse|Mouse|Rat|Mouse|Mouse|Xenopus|Human|Rat|Mouse|Mouse|Mouse|Mouse|Rat|Mouse|Mouse|Xenopus laevis|Mouse|Mouse|Rat|Hamster|Mouse
Contact our support service to receive datasheet or other technical documentation.
Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody (SPC-405). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-405) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green). | Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-405). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-405) at 1:1000. | Western blot analysis of Mouse kidney cortex showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-405). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-405) at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-7). 70kDa degradation band observed in low-salt. Rabbit Anti-ENaC Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-405) | Rabbit Anti-ENaC Antibody used in Western blot (WB) on Rat kidney tissue lysates (SPC-405) | Rabbit Anti-ENaC Antibody used in Western blot (WB) on Mouse kidney cortex (SPC-405)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Oryctolagus cuniculus,Rattus norvegicus