Immunogen
Synthetic peptide mapping to AA 809 to 831 of rat sequence
Antibody's target
Rat NHE3
Antibody's full description
Rabbit Anti-Rat NHE3 Polyclonal
100 µg
SPC-400D-ALP
400€
Synthetic peptide mapping to AA 809 to 831 of rat sequence
Rat NHE3
Rabbit Anti-Rat NHE3 Polyclonal
Cell Signaling, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters, Cancer
Polyclonal Antibodies
SLC9A3 Antibody, Na+/H+ exchanger 3 Antibody, isoform 3 Antibody, Solute carrier family 9 member 3 Antibody, NHE3MGC126720 Antibody, MGC126718 Antibody, solute carrier family 9 (sodium/hydrogen exchanger) member 3 Antibody, NHE-3 Antibody, Na(+)/H(+) exchanger 3 Antibody, Sodium/hydrogen exchanger 3 Antibody
WB, ICC/IF
Rabbit
Rat, Mouse
WB (1:1000); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~84kDa.
-20°C
Blue Ice or 4°C
1 µg/ml of SPC-400 was sufficient for detection of HNE3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Membrane
See included datasheet or contact our support service.
Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).
1. Kim G.H., Ecelbarger C.A., Knepper M.A. and Packer R.K. (1999) J Am Soc Nephrol. 10: 935-942. 2. Dynia D.W., Steinmetz A.G. and Kocinsky H.S. (2010) Am J Physoil Renal Physiol. 298(3): F745-F753.
11/Jun/2010
NP_036786
24784
P26433
27443990|26839400|26224717|25925252|23281477
Western Blot|Western Blot||Western Blot|Western Blot
Rat|Mouse||Mouse|Mouse
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Rat kidney tissue lysates showing detection of NHE3 protein using Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400). Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400) at 1:1000. Rabbit Anti-NHE3 Antibody used in Western blot (WB) on Rat kidney tissue lysates (SPC-400)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Oryctolagus cuniculus,Rattus norvegicus
