Immunogen
Peptide derived from C-terminal region of human LC3B protein
Antibody's target
Human LC3B
Antibody's full description
Rabbit Anti-Human LC3B Polyclonal
50 µg
SPC-217A-ALP
381€
Peptide derived from C-terminal region of human LC3B protein
Human LC3B
Rabbit Anti-Human LC3B Polyclonal
Cancer, Autophagy, Cell Signaling, Neuroscience, Cardiovascular System, Heart
Polyclonal Antibodies
MAP1LC3B Antibody, Autophagy related protein LC3 B Antibody, Autophagy related ubiquitin like modifier LC3 B Antibody, Autophagy-related protein LC3 B Antibody, Autophagy-related ubiquitin-like modifier LC3 B Antibody, LC3 Antibody, MAP1 light chain 3 like protein 2 Antibody, MAP1 light chain 3-like protein 2 Antibody, MAP1A/1B light chain 3 B Antibody, MAP1A/1BLC3 Antibody, Antibody, MAP1A/MAP1B LC3 B Antibody, MAP1A/MAP1B light chain 3 B Antibody, Antibody, MAP1BLC3 Antibody, Antibody, Map1lc3b Antibody, Antibody, Microtubule associated protein 1 light chain 3 beta Antibody, Microtubule associated proteins 1A/1B light chain 3 Antibody, Microtubule associated proteins 1A/1B light chain 3A Antibody, Microtubule associated proteins 1A/1B light chain 3B Antibody, Microtubule-associated protein 1 light chain 3 beta Antibody, Microtubule-associated proteins 1A/1B light chain 3B Antibody, MLP3B_HUMAN Antibody
WB
Rabbit
Human, Mouse, Rat
WB (1:1000); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS, 15mM sodium azide
See included datasheet or contact our support service
Specific for LC3B. Does not detect LC3A, LC3C, GABARAP, GABARAPL1 or GABARAPL2. Detects LC3B-I and LC3B-II.
4°C
Blue Ice or 4°C
A 1:10000 dilution of SPC-217 was sufficient for detection of LC3B in lysate from cells expressing Recombinant GST-tagged LC3B by electrochemiluminescence analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Cytoskeleton, Endomembrane System, Lipid-anchor, Cytoplasmic Vesicle, Autophagosome, Autophagosome Membrane
See included datasheet or contact our support service.
Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.
1. Tanida I., Ueno T., & Kominami E. (2008) Methods Mol Biol. 445:77-88. 2. He H., et al. (2003) J Biol Chem. 278(31): 29278-87.
12/Jun/2014
NP_073729.10
81640
Q9GZQ17
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.05
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
