Immunogen
Human 20S proteasome extracted from erythrocytes
Antibody's target
Human 20S Proteasome
Antibody's full description
Rabbit Anti-Human 20S Proteasome Polyclonal
100 µl
SPC-215D-ALP
381€
Human 20S proteasome extracted from erythrocytes
Human 20S Proteasome
Rabbit Anti-Human 20S Proteasome Polyclonal
Cancer, Apoptosis
Polyclonal Antibodies
20S core particle Antibody, Macropain Antibody, Multicatalytic endopeptidase complex subunit Antibody, Prosome subunit Antibody, Proteasome subunit alpha type 1 Antibody, Proteasome subunit alpha type 5 Antibody, Proteasome subunit alpha type 7 Antibody, Proteasome subunit Antibody, Proteasome subunit beta type 1 Antibody, Proteasome subunit beta type 5 Antibody, Proteasome subunit beta type 6 Antibody, Proteasome subunit beta type 7 Antibody, PSMA1 Antibody, PSMA5 Antibody, PSMA7 Antibody, PSMB1 Antibody, PSMB5 Antibody, PSMB6 Antibody, PSMB7 Antibody, ZETA Antibody
WB, ICC/IF, IP
Rabbit
Human, Mouse
WB (1:5000); optimal dilutions for assays should be determined by the user.
Rabbit Antiserum
Rabbit Antiserum, 0.1% sodium azide
See included datasheet or contact our support service
Detects multiple 20S proteasome subunits.
4°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-215 was sufficient for detection of 20S proteasome ‘core’ subunits in 0.1 µg of human 26S proteasome by electrochemiluminescence analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm
See included datasheet or contact our support service.
Proteasomes function in the degradation of unusable or damaged cellular proteins. Proteasome complexes are therefore localized in the nucleus and cytoplasm of eukaryotic cells. The most commonly found proteasome in mammalian cytosol is the 26S proteasome, which is made up of one 20S proteasome core subunit and two 19S subunits. The 20S core particle is in itself made up of two alpha subunits and two beta subunits, which represent the catalytic component. Proteins destined for degradation are first labeled by polyubiquitination before they enter the proteasome core and are broken down.
1. Saeki Y., & Tanaka K. (2012) Methods Mol Biol. 832: 315-37. 2. Geng F., Wenzel S., & Tansey W. (2012) Annu Rev Biochem. 81:177-201.
12/Jun/2014
Refer to NCBI
Refer to GenBank
Refer to Swiss-Prot
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
