Immunogen
Rat native full-length HO-1 purified from liver tissue
Antibody's target
Rat HO-1
Antibody's full description
Rabbit Anti-Rat HO-1 Polyclonal
100 µg
SPC-211D-ALP
391€
Rat native full-length HO-1 purified from liver tissue
Rat HO-1
Rabbit Anti-Rat HO-1 Polyclonal
Cancer, Oxidative Stress
Polyclonal Antibodies
Heme oxygenase 1 Antibody, HSP32 Antibody, Hemox Antibody, 32 kD Antibody, bK286B10 Antibody, D8Wsu38e Antibody, heat shock protein 32kD Antibody, Heat shock protein Antibody, Heme oxygenase (decycling) 1 Antibody, HMOX 1 Antibody, Hmox Antibody, HMOX1 Antibody, HO 1 Antibody, HO Antibody, HO1 antibody
WB, IHC, IP
Rabbit
Human, Mouse, Rat
WB (1:400), IHC (1:1000), IP (1:100); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS pH7.4, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~33kDa.
-20°C
Blue Ice or 4°C
5 µg/ml of SPC-211 was sufficient for detection of HO-1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Endoplasmic Reticulum, Microsome
See included datasheet or contact our support service.
Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.
7/May/2014
NP_036712.1
24451
P06762
Refer to PubMed
To be tested
Immunohistochemistry analysis using Rabbit Anti-HO-1 Polyclonal Antibody (SPC-211). Tissue: Brain. Species: Rat. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody (SPC-211) at 1:1000. | Western blot analysis of Rat Brain cell lysates showing detection of HO-1 protein using Rabbit Anti-HO-1 Polyclonal Antibody (SPC-211). Lane 1: Rat Brain lysate. Lane 2: Purified HO-1. Lane 3: Molecular Weight Markers. Load: 10 µg. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody (SPC-211) at 1:1000. Rabbit Anti-HO-1 Antibody used in Immunohistochemistry (IHC) on Rat Brain (SPC-211) | Rabbit Anti-HO-1 Antibody used in Western blot (WB) on Rat Brain cell lysates (SPC-211)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Oryctolagus cuniculus,Rattus norvegicus
