Immunogen
Human recombinant TRAP1 (amino acids 1-59 removed - transit peptide)
Antibody's target
Human TRAP1
Antibody's full description
Rabbit Anti-Human TRAP1 Polyclonal
100 µg
SPC-209D-ALP
369€
Human recombinant TRAP1 (amino acids 1-59 removed - transit peptide)
Human TRAP1
Rabbit Anti-Human TRAP1 Polyclonal
Cancer, Heat Shock, Cell Signaling, Trafficking
Polyclonal Antibodies
Heat shock protein 75 Antibody, Heat shock protein 75 kDa Antibody, Heat shock protein 75 kDa mitochondrial Antibody, HSP 75 Antibody, HSP 90L Antibody, HSP75 Antibody, HSP90L Antibody, mitochondrial Antibody, TNF receptor associated protein 1 Antibody, TNFR associated protein 1 Antibody, TNFR-associated protein 1 Antibody, TRAP 1 Antibody, TRAP-1 Antibody, Trap1 Antibody, TRAP1_HUMAN Antibody, Tumor necrosis factor receptor associated protein Antibody, Tumor necrosis factor type 1 receptor associated protein Antibody, Tumor necrosis factor type 1 receptor-associated protein Antibody
WB, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:1000), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS pH7.4, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~75 kDa, multiple isoforms.
-20°C
Blue Ice or 4°C
1 µg/ml of SPC-209 was sufficient for detection of Trap-1 in 20 µg of Hela Cell lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Mitochondrion
See included datasheet or contact our support service.
The 90 kDa heat shock protein (HSP90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. HSP90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of HSP90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like HSP90, TRAP1 is found to beassociated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).
1. Felts S.J., et al. (2000) J Biol Chem. 275(5): 3305-3312. 2. Costantino E., et al. (2009) Cancer Lett. 279(1): 39-46. 3. Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.
25/Sep/2013
NP_057376.2
10131
Q12931
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TRAP1 Polyclonal Antibody (SPC-209). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody (SPC-209) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Human Cervical Cancer cell lysates (HeLa) showing detection of ~75 kDa TRAP1 protein using Rabbit Anti-TRAP1 Polyclonal Antibody (SPC-209). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical Cancer cell lysates (HeLa). Load: 12 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody (SPC-209) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~75 kDa. Other Band(s): 100 kDa. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TRAP1 Polyclonal Antibody (SPC-209). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody (SPC-209) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Rabbit Anti-TRAP1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-209) | Rabbit Anti-TRAP1 Antibody used in Western blot (WB) on Cervical Cancer cell lysates (HeLa) (SPC-209) | Rabbit Anti-TRAP1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-209)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
