Immunogen
Yeast Aha1 raised against peptide (CESQVKSNYTRGNQK)-KLH fusion
Antibody's target
Yeast AHA1p
Antibody's full description
Rabbit Anti-Yeast AHA1p Polyclonal
100 µl
SPC-204D-ALP
369€
Yeast Aha1 raised against peptide (CESQVKSNYTRGNQK)-KLH fusion
Yeast AHA1p
Rabbit Anti-Yeast AHA1p Polyclonal
Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
Polyclonal Antibodies
Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody, Aha1 Antibody, SHSA1 Antibody, HSPC322 Antibody, p38 Antibody
WB
Rabbit
Yeast
WB (1:2500); optimal dilutions for assays should be determined by the user.
Rabbit Antiserum
Rabbit Antiserum, PBS, 50% glycerol
See included datasheet or contact our support service
Detects ~ 38kDa. Can run up to 45kDa on SDS Page.
-20°C
Blue Ice or 4°C
0.5 µg/ml of SPC-204 was sufficient for detection of Aha1p in 20 µg of W303 yeast lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Endoplasmic Reticulum
See included datasheet or contact our support service.
Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
2/Apr/2013
NP_010500.3
851800
Q12449
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
