Immunogen
Yeast Sti1p raised against peptide(CDINQSNSMPKEPET)-KLH fusion
Antibody's target
Yeast HOP
Antibody's full description
Rabbit Anti-Yeast STI1P (HOP) Polyclonal
100 µl
SPC-203D-ALP
369€
Yeast Sti1p raised against peptide(CDINQSNSMPKEPET)-KLH fusion
Yeast HOP
Rabbit Anti-Yeast STI1P (HOP) Polyclonal
Cancer, Heat Shock
Polyclonal Antibodies
STIP1 Antibody, Sti1 yeast Antibody, Hop Antibody, HSC70/HSP90 organizing protein Antibody, Epididymis secretory sperm binding protein Li 94n antibody, HEL S 94n antibody, Hop antibody, HSC70/HSP90 organizing protein antibody, HSC70/HSP90-organizing protein antibody, IEF SSP 3521 antibody, NY REN 11 antigen antibody, P60 antibody, Renal carcinoma antigen NY-REN-11 antibody, STI1 antibody, STI1L antibody, STIP1 antibody, Stress induced phosphoprotein 1 antibody, Stress-induced-phosphoprotein 1 antibody, Transformation sensitive protein IEF SSP 3521 antibody, Transformation-sensitive protein IEF SSP 3521 antibody
WB, ELISA, FCM
Rabbit
Mouse, Yeast
WB (1:2500); optimal dilutions for assays should be determined by the user.
Rabbit Antiserum
Rabbit Antiserum, PBS, 50% glycerol
See included datasheet or contact our support service
Detects ~ 66kDa.
-20°C
Blue Ice or 4°C
0.5 µg/ml of SPC-203 was sufficient for detection of Sti1p (HOP) in 20 µg of W303 yeast lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus
See included datasheet or contact our support service.
The stress inducible protein Sti1p is also commonly known as HSP70-HSP90 organization protein (HOP). It is located in diverse cellular regions and can move between the cytoplasm and the nucleus. It functions to reversibly link together the protein chaperones HSP70 and HSP90. HOP contains three tetratricopeptide repeat (TPR) domains, TPR1, TPR2a and TPR2b. HSP70 binding has been localized to TRP1 and sp90 binding have been localized to TPR2a (1). It has also been found to modulate the chaperone activities of the linked proteins and possible interacts with other chaperones and proteins. It has also been found to participate in other complexes besides the HSP70/HSP90 one (2). HOP is closely related to human 63kDa protein that is sensitive to simian virus SV40 transformation, and is related to the yeast heat-shock- responsive STI1 gene product (3, 4).
1. Flom G., Behal R.H., Rosen L., Cole D.G., Johnson J.L. (2007) Biochem J. 404(1): 159-167. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt3): 789-796. 3. Honore B.H., et al. (1992) J Biol Chem. 267: 8485- 8491. 4. Nicolet C.M., et al. (1989) Mol Cell Bio. 9: 3638-3646.
2/Apr/2013
NP_014670.1
854192
P15705
27665558|27665558
ELISA|Flow Cytometry
Mouse|Mouse
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
