Immunogen
Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
Antibody's target
Human TLR4
Antibody's full description
Rabbit Anti-Human TLR4 Polyclonal
100 µg
SPC-200D-ALP
359€
Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
Human TLR4
Rabbit Anti-Human TLR4 Polyclonal
Cell Signaling, Cardiovascular System, Atherosclerosis
Polyclonal Antibodies
ARMD10 Antibody, CD284 Antibody, TLR 4 Antibody, TOLL Antibody, Toll like receptor 4 Antibody
WB, IHC, ICC/IF, FCM, FACS
Rabbit
Human, Mouse
WB (1:500), IHC (1:50); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
-20°C
Blue Ice or 4°C
2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Membrane
See included datasheet or contact our support service.
The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).
1. Muzio M., Natoli G., Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101. 2. Medzhitov R. and Janeway C.A (1997) Cell. 91: 295-298. 3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta. 1518(1-2): 157-161. 4. Takeuchi O., et al. (1999) Immunity. 11: 443. 5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA. 97: 2163-2167. 6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature. 388(6640): 394-397.
1/Jan/2011
NP_612564.1
7099
O00206
26235132|25595264
Western Blot|Immunohistochemistry
Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: colon colitis. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Localization: Inflammatory cells. | Western blot analysis of Human, Mouse Cell line lysates showing detection of TLR4 protein using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:1000. | Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: Spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100. | Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100. Rabbit Anti-TLR4 Antibody used in Immunohistochemistry (IHC) on Mouse colon colitis (SPC-200) | Rabbit Anti-TLR4 Antibody used in Western blot (WB) on Human, Mouse Cell line lysates (SPC-200) | Rabbit Anti-TLR4 Antibody used in Immunohistochemistry (IHC) on Mouse Spleen tissue (SPC-200) | Rabbit Anti-TLR4 Antibody used in Immunohistochemistry (IHC) on Mouse spleen tissue (SPC-200)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
