Immunogen
NM_003458.3 (AA 786-1041) N-terminal his-tagged fusion protein
Antibody's target
Human Bassoon
Antibody's full description
Rabbit Anti-Human Bassoon Polyclonal
100 µg
SPC-198D-ALP
390€
NM_003458.3 (AA 786-1041) N-terminal his-tagged fusion protein
Human Bassoon
Rabbit Anti-Human Bassoon Polyclonal
Neuroscience, Cell Structure, Pre-Synaptic Markers
Polyclonal Antibodies
BSN Antibody, ZNF231 Antibody, Neuronal double zine finger protein Antibody
WB, IHC
Rabbit
Human, Mouse, Rat
WB (1:1000), IHC (1:400); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS pH7.4, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~420kDa. Multiple isoforms can be detected.
-20°C
Blue Ice or 4°C
1 µg/ml of SPC-198 was sufficient for detection of Bassoon in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Cell Junction, Synapse
See included datasheet or contact our support service.
Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntington, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to co-localize with GABA (A) and glutamate (GluR1) receptors (1).
1. Anna Dondzillo et al., (2010) J. Comp Neur, 518(7): 1008–1029.
22/Mar/2011
NP_003449.2
8927
Q9UPA5
Refer to PubMed
To be tested
Immunohistochemistry analysis using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ. | Western blot analysis of Mouse, Rat brain cell lysates showing detection of Bassoon protein using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:1000. Rabbit Anti-Bassoon Antibody used in Immunohistochemistry (IHC) on Mouse Muscle (SPC-198) | Rabbit Anti-Bassoon Antibody used in Western blot (WB) on Mouse, Rat brain cell lysates (SPC-198)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
