Immunogen
Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse
Antibody's target
Hamster HSP110
Antibody's full description
Rabbit Anti-Hamster HSP110 Polyclonal
100 µg
SPC-195D-ALP
341€
Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse
Hamster HSP110
Rabbit Anti-Hamster HSP110 Polyclonal
Cancer, Heat Shock
Polyclonal Antibodies
Heat shock 105kDa alpha Antibody, heat shock 105kDa beta Antibody, HSP105 Antibody, HSP105A Antibody, HSP105B Antibody, HSP110 Antibody, HSPH1 Antibody, NY CO 25 Antibody
WB, IHC
Rabbit
Human, Mouse, Rat, Bovine, Monkey, Hamster, Sheep, Yeast, Shark, Gummy Shark (Mustelus antarcticus), School Shark (Galeorhinus galeus)
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS pH7.2, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~110kDa.
-20°C
Blue Ice or 4°C
1 µg/ml of SPC-195 was sufficient for detection of HSP110 in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm
See included datasheet or contact our support service.
HSP110 belongs to a family of large stress proteins known as the HSP110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied HSP70 family. They share 30-33% amino acid identity, mostly in the conserved ATP-binding domain (1). HSP110 cooperates with HSP70 in protein folding in the eukaryotic cytosol (2). In mammals, HSP110 is constitutively expressed, but exhibits particularly high levels in the brain. Both HSP70 and HSP110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of HSP110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of HSP70 (3). Studies also suggest that HSP110 can be used in heat shock protein-based cancer immunotherapy (4).
1. Lee-Yoon D., et al. (1995) J Biol Chem. 270: 15725-15733. 2. Dragovic Z., et al. (2006) EMBO J. 25: 2519-2528. 3. Nakamura J., et al. (2008) Stroke. 39(10): 2853-2859. 4. Subject D.R. (2001) Health Research Inc. Report number AS90893: 1-84.
23/Feb/2010
NP_006635.3
10808
Q92599
27706135|27207636|25430986
Western Blot|Western Blot|Western Blot
Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining. | Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. Rabbit Anti-HSP110 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-195) | Rabbit Anti-HSP110 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-195)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
