Immunogen
Human Cpn10 peptide AA 91-101
Antibody's target
Human Cpn10
Antibody's full description
Rabbit Anti-Human Cpn10 Polyclonal
100 µg
SPC-193D-ALP
311€
Human Cpn10 peptide AA 91-101
Human Cpn10
Rabbit Anti-Human Cpn10 Polyclonal
Cancer, Heat Shock
Polyclonal Antibodies
10kDa Chaperonin Antibody, Chaperonin 10 Antibody, Cpn 10 Antibody, EPF Antibody, GROES Antibody, Heat Shock 10kD protein1 Antibody, HSP10 Antibody, HSPE1 Antibody
WB, IHC, ICC/IF, IP, ELISA
Rabbit
Human, Mouse, Rat, Bovine, Dog, Guinea Pig (Cavia porcellus), Pig, Rabbit, Sheep, African clawed frog (Xenopus laevis)
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS pH7.3, 0.02% sodium azide
1 mg/ml
Detects ~ 10kDa.
4°C
Blue Ice or 4°C
1 µg/ml of SPC-193 was sufficient for detection of Cpn10 in 10 µg of Hela lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Mitochondrion, Mitochondrion Matrix
See included datasheet or contact our support service.
Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kDa (HSP10s). Cpn10 acts as a co-chaperone and interacts with the HSP60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process (1). Cpn10 has been located in human platelets, but is also present in human maternal serum (2, 3). It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy (4).
1. Velez-Granell C.S., et al. (1994) J of Cell Science. 107(3): 539-549. 2. Morton H., Hegh V., and Clunie G.J.A. (1974) Nature (London) 249: 459-460. 3. Cavanagh A.C., and Morton H. (1994) Eur. J. Biochem. 222: 551-560. 4. Minto M., et al. (1998) Molecular Cell Research. 1403 (2): 151-157.
1/Dec/2009
NP_002148.1
3336
P61604
Refer to PubMed
To be tested
Immunohistochemistry analysis using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis.. | Western blot analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (SPC-193) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. Rabbit Anti-Cpn10 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-193) | Rabbit Anti-Cpn10 Antibody used in Western blot (WB) on Rat brain cell lysates (SPC-193)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
