Immunogen
C-terminal peptide of Pf11_0513 conjugated to KLH
Antibody's target
P. falciparum HSP40
Antibody's full description
Rabbit Anti-P. Falciparum HSP40 Polyclonal
100 µg
SPC-184D-ALP
390€
C-terminal peptide of Pf11_0513 conjugated to KLH
P. falciparum HSP40
Rabbit Anti-P. Falciparum HSP40 Polyclonal
Cancer, Heat Shock
Polyclonal Antibodies
DNAJ1 Antibody, NDAJB1 Antibody, HDJ1 Antibody, HSP40 Antibody, HSPF1 Antibody, DnaJ homolog subfamily B member 1 Antibody, Dna J protein homolog 1 Antibody, Heat shock 40 kDa protein 1 Antibody, HSP40 Antibody, heat shock protein 40 Antibody, Human DnaJ protein 1 Antibody, hDj-1 Antibody
WB, ICC/IF
Rabbit
Plasmodium falciparum
WB (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS pH7.4, 50% glycerol, 0.09% sodium azide
1.8 mg/ml
Detects ~ 62kDa. Specific to P. Falciparum and does not cross-react to any protein from Human erythrocytes.
-20°C
Blue Ice or 4°C
0.9 µg/ml of SPC-184 was sufficient for detection of Pf11_0513 in 20 µg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus
See included datasheet or contact our support service.
DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s. HSP40, also known as HDJ1 (1), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-6). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (3). PF11_0513 belongs to the HSP40 family of chaperones. This protein has a Plasmodium export element (PEXEL). It is exported out of the parasite into the infected erythrocytic compartment.
1. Ohtsuka K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240. 2. Melville M.W., et al. (1997) PNAS USA. 94: 97-102. 3. Hattori H., Let al. (1992) Cell Structure and Function. 17: 77-86. 4. Ohtsuka K. Masuda A., Nakai A., and Nagata K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647. 5. Bardwell J.C.A., et al. (1986) J. Biol. Chem. 261: 1782-1785. 6. Ohku M., Tamura F., Nishimura S., and Uchida H. (1986) J. Biol. Chem. 261: 1778-1781.
27/Apr/2009
XP_001348169.3
811046
Q8IHM7
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Parasite lysates showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184). Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody (SPC-184) at 1:2000. Rabbit Anti-HSP40 Antibody used in Western blot (WB) on Parasite lysates (SPC-184)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
