Immunogen
Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Antibody's target
Dog Calnexin
Antibody's full description
Rabbit Anti-Dog Calnexin-CT Polyclonal
200 µg
SPC-182B-ALP
472€
Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Dog Calnexin
Rabbit Anti-Dog Calnexin-CT Polyclonal
Cell Signaling, Organelle Markers
Polyclonal Antibodies
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
WB, IHC, ICC/IF, IP, FCM
Rabbit
Human, Mouse, Rat, Bovine, Monkey, Chicken, Dog, Guinea Pig (Cavia porcellus), Hamster, Pig, Avian, Quail, Rabbit, Sheep, Fruit Fly (Drosophila melanogaster), African clawed frog (Xenopus laevis)
WB (1:2000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS pH7.2, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects the C-terminal domain of Calnexin ~90kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.
-20°C
Blue Ice or 4°C
A 1:2000 dilution of SPC-182 was sufficient for detection of Calnexin in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Melanosome
See included datasheet or contact our support service.
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science. 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry. 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell. 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.
23/Mar/2011
NP_001003232.1
403908
P24643
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182) at 1:80 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Melanosome. Magnification: 100x. Heat Shocked at 42°C for 1h. | Western blot analysis of Rat tissue mix showing detection of Calnexin-CT protein using Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunohistochemistry analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Hair Follicles, Basal cells in epidermis, and second layer of epidermis. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear staining, cytoplasmic staining. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182) at 1:80 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Calnexin-CT Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Rabbit Anti-Calnexin-CT Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-182) | Rabbit Anti-Calnexin-CT Antibody used in Western blot (WB) on Rat tissue mix (SPC-182) | Rabbit Anti-Calnexin-CT Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-182) | Rabbit Anti-Calnexin-CT Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-182) | Rabbit Anti-Calnexin-CT Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-182)
Non-hazardous
Canada
1.4
0.2
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Canine or Canis Lupus is mostly Beagle used for drug research. StressMark antibodies produces ELISA test kits and polyclonal antibodies.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
