Immunogen
Human HSP22
Antibody's target
Human HSP22
Antibody's full description
Rabbit Anti-Human HSP22 Polyclonal
100 µl
SPC-181D-ALP
341€
Human HSP22
Human HSP22
Rabbit Anti-Human HSP22 Polyclonal
Cancer, Heat Shock
Polyclonal Antibodies
Alpha crystallin C chain Antibody, CMT2L Antibody, CRYAC Antibody, DHMN2 Antibody, H11 Antibody, Heat shock 22kDa protein 8 Antibody, HMN2 Antibody, HSB8 Antibody, HSPB8 Antibody
WB, IHC, ICC/IF, IP
Rabbit
Human, Mouse, Rat
WB (1:1000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~22kDa. Does not cross-react with HSP27 or alpha-crystallin.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-181 was sufficient for detection of HSP22 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus
See included datasheet or contact our support service.
HSP22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to HSP27. Similar to most other sHSPs, HSP22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). HSP22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of HSP22 in various processes including proliferation, apoptosis and macro autophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).
1. Kappe G., et al. (2001) Biochem Biophys Acta. 1520: 1-6. 2. Benndorf R., et al. (2001) J Biol Chem. 276: 26753-26761. 3. Sun X., et al. (2004) J Biol Chem. 279: 2394-2402. 4. Kim M.V., et al. (2004) Biochem Biophys Res Commun. 325: 649-652. 5. Wilhelmus M.M., et al. (2006) Acta Neuropathol. (Berl) 111: 139-149.
23/Mar/2011
NP_055180.2
26354
Q9UJY2
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear Staining. | Western blot analysis of Rat Skeletal muscle lysates showing detection of HSP22 protein using Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunohistochemistry analysis using Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Epidermis positive, dermal staining. | Immunohistochemistry analysis using Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181). Tissue: Spinal cord. Species: Mouse. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181) at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. DAPI merged with Alexa 488. Courtesy of: Joy Irobi-Devolder, University of Antwerp, Belgium.. Rabbit Anti-HSP22 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-181) | Rabbit Anti-HSP22 Antibody used in Western blot (WB) on Rat Skeletal muscle lysates (SPC-181) | Rabbit Anti-HSP22 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-181) | Rabbit Anti-HSP22 Antibody used in Immunohistochemistry (IHC) on Mouse Spinal cord (SPC-181)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
