Immunogen
Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3Beta
Antibody's target
Human GSK3 Beta
Antibody's full description
Rabbit Anti-Human GSK-3beta Polyclonal
100 µg
SPC-169D-ALP
342€
Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3Beta
Human GSK3 Beta
Rabbit Anti-Human GSK-3beta Polyclonal
Cell Signaling, Post-Translational Modifications, Phosphorylation
Polyclonal Antibodies
Glycogen Synthase kinase 3 beta Antibody, GSK 3 beta Antibody, GSK 3B Antibody, GSK-3 beta Antibody, GSK3B Antibody, GSK3B_Human Antibody, GSK3beta isoform Antibody
WB, IHC, ICC/IF
Rabbit
Human, Mouse, Rat, Bovine
WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS pH7.4
1.3 mg/ml
Detects ~47kDa.
4°C
Blue Ice or 4°C
1 µg/ml of SPC-169 was sufficient for detection of GSK-beta in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus, Cell Membrane
See included datasheet or contact our support service.
Glycogen synthase kinase 3, is a serine/threonine protein kinase that mediates the addition of phosphate molecules onto serine and threonine amino acid residues. It has also been shown to regulate immune and migratory processes, and is tied to pathways of cell proliferation and apoptosis (1-3). In mammals, it is encoded by two genes- GSK-3Alpha and GSK-3Beta. They are structurally similar, but functionally non-identical. GSK-3Alpha is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3Beta negatively regulates cardiac hypertrophy and cardiac development through its effect on WNT signaling (4). GSK-3 alpha and GSK-3 beta share 85% amino acid identity. GSK-3 has been implicated in a number of diseases including Type II diabetes, Alzheimer’s, cancer and bipolar (4, 5).
1. Jope R.S. (2007) Neurochem Res. 32(4-5): 577-595. 2. Wang H. (2011) Cytokind. 53(2): 130-140. 3. Mills C.N. (2011) Front Mol Neurosci. 47(4): 47. 4. Hardt S.E., Sadoshima J. (2002) Circ Res. 90(10): 1055-1063. 5. Doble D.W., Woodgett J.R. (2003) J Cell Sci. 116(Pt7): 1175-1186.
25/Jul/2012
NP_001139628.1
2932
P49841
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GSK3beta Polyclonal Antibody (SPC-169). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GSK3beta Polyclonal Antibody (SPC-169) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GSK3beta Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Human Cell line lysates showing detection of GSK3 Beta protein using Rabbit Anti-GSK3 Beta Polyclonal Antibody (SPC-169). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-GSK3 Beta Polyclonal Antibody (SPC-169) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GSK3beta Polyclonal Antibody (SPC-169). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GSK3beta Polyclonal Antibody (SPC-169) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GSK3beta Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Rabbit Anti-GSK3beta Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-169) | Rabbit Anti-GSK3 Beta Antibody used in Western blot (WB) on Human Cell line lysates (SPC-169) | Rabbit Anti-GSK3beta Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-169)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
