Immunogen
Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues
Antibody's target
Human Rab5
Antibody's full description
Rabbit Anti-Human Rab5 Polyclonal
100 µg
SPC-168D-ALP
330€
Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues
Human Rab5
Rabbit Anti-Human Rab5 Polyclonal
Cell Signaling, Organelle Markers, Trafficking, Neuroscience, Cell Structure, Pre-Synaptic Markers
Polyclonal Antibodies
Rab 5A Antibody, RAS associated protein RAB5A Antibody, Ras related protein Rab 5 A Antibody
WB, IHC, ICC/IF
Rabbit
Human, Mouse, Rat, Bovine, Monkey
WB (1:1000), IHC (1:100), ICC/IF (1:80); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~26kDa.
-20°C
Blue Ice or 4°C
1 µl/ml of SPC-168 was sufficient for detection of Rab5 in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
Cell Membrane, Endosome, Early Endosome Membrane, Melanosome
See included datasheet or contact our support service.
Rab5 is a 24kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.
1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Sonnichsen B., et al. (2000) J. Cell Biol. 149: 901-913 6. Woodman P.G. (2000) Traffic. 1: 695-701.
13/Aug/2009
NP_004153.2
5868
Q6FI44
21586748
Immunocytochemistry/Immunofluorescence
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168) at 1:80 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody. (C) Composite. | Western blot analysis of Human Cell line lysates showing detection of Rab5 protein using Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunohistochemistry analysis using Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168) at 1:80 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody. (C) Composite. Rabbit Anti-Rab5 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-168) | Rabbit Anti-Rab5 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-168) | Rabbit Anti-Rab5 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-168) | Rabbit Anti-Rab5 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-168)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
