Immunogen
His-tagged rat GRP78
Antibody's target
Rat GRP78
Antibody's full description
Rabbit Anti-Rat GRP78 Polyclonal
100 µg
SPC-167D-ALP
364€
His-tagged rat GRP78
Rat GRP78
Rabbit Anti-Rat GRP78 Polyclonal
Cancer, Heat Shock, Cell Signaling, Organelle Markers, Trafficking, Chaperones
Polyclonal Antibodies
BIP Antibody, Grp78 Antibody, HSPA5 Antibody, MIF2 Antibody, immunoglobulin heavy chain binding protein Antibody
WB, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:1000), ICC/IF (1:60); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS pH7.4, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~78kDa. Weak detection in human tissues.
-20°C
Blue Ice or 4°C
1 µg/ml of SPC-167 was sufficient for detection of Grp78 in 10 µg of rat brain cell lysate by colorimetric immunoblot analysis using Goat anti -rabbit IgG:HRP as the secondary antibody.
Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Melanosome
See included datasheet or contact our support service.
GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti¬tumor drugs (6, 7).
1. Cho S., et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo S., et al (2006) 26 (15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19:4333- 4336. 6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res 65(13): 5785-91.
27/Mar/2013
NP_037215.1
25617
P06761.1
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167) at 1:60 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167) at 1:60 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Rat Brain cell lysates showing detection of ~78 kDa GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~78 kDa. Rabbit Anti-GRP78 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-167) | Rabbit Anti-GRP78 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-167) | Rabbit Anti-GRP78 Antibody used in Western blot (WB) on Brain cell lysates (SPC-167)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Oryctolagus cuniculus,Rattus norvegicus
