Immunogen
Methylated KLH Conjugated
Antibody's target
Methylated Lysine
Antibody's full description
Rabbit Anti-Methylated Lysine Polyclonal
400 µl
SPC-158F-ALP
400€
Methylated KLH Conjugated
Methylated Lysine
Rabbit Anti-Methylated Lysine Polyclonal
Cell Signaling, Post-Translational Modifications, Methylation
Polyclonal Antibodies
Dimethylysine Antibody, Methyl lysine Antibody, N epsilon dimethyl lysine Antibody, Trimethyl lysine Antibody
WB, ICC/IF, IP, ELISA
Rabbit
Species Independent
WB (1:5000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS, 50% glycerol
0.25 mg/ml
Detects proteins containing methylated lysine residues.
-20°C
Blue Ice or 4°C
0.2-0.5 µg/ml of SPC-158 was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.
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See included datasheet or contact our support service.
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
1. Yang X.J. (2005) Oncogene. 24:1653-1662. 2. Hassig C.A. and Schreiber S.L. (1997) Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang X.J. (2004) Bioessays 26:1076-1087. 4. Hughes R.E. (2002) Curr. Biol. 12: R141-R143. 5. Vigushin D.M. and Coombes R.C. (2004) Curr. Cancer Drug Targets 4: 205-218. 6. Chan H.M. et al. (2001) Nat. Cell Biol. 3: 667-674. 7. Martinez-Balbas M.A. et al. (2000) EMBO J. 19: 662-671.
1/Jun/2007
Refer to NCBI
Refer to GenBank
Refer to Swiss-Prot
27858264|27521690|25609799|25374779|24651940|24233354|23533717
Dot Blot|Western Blot|Western Blot|Western Blot|Dot Blot|Dot Blot|Dot Blot
Otala lactea (milk snail)|Squirrel|Hamster |Ictidomys tridecemlineatus (ground squirrel)|Xenopus laevis|Littorinalittorea (intertidal marine snail)|Turtle
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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Methylated Lysine Antibody. (C) Composite. | Western blot analysis of Bovine serum albumin showing detection of Methylated Lysine protein using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:1000. Methylated Lysine in BSA (Left) and Methylated BSA (Right). Rabbit Anti-Methylated Lysine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-158) | Rabbit Anti-Methylated Lysine Antibody used in Western blot (WB) on Bovine serum albumin (SPC-158)
Non-hazardous
Canada
1.4
0.4
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus