Immunogen
Phosphothreonine conjugated to KLH
Antibody's target
Phosphothreonine
Antibody's full description
Rabbit Anti-Phosphothreonine Polyclonal
400 µl
SPC-154F-ALP
400€
Phosphothreonine conjugated to KLH
Phosphothreonine
Rabbit Anti-Phosphothreonine Polyclonal
Cell Signaling, Post-Translational Modifications, Phosphorylation
Polyclonal Antibodies
Phospho-threonine Antibody
WB, ICC/IF, IP, ELISA
Rabbit
Species Independent
WB (1:500), ICC/IF (1:60), ELISA (1:2000), IP (1:100); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS, 50% glycerol, 0.09% sodium azide
0.25 mg/ml
Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.
-20°C
Blue Ice or 4°C
2 µg/ml of SPC-154 was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium.
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See included datasheet or contact our support service.
Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Ostrovsky P.C. (1995) Genes Dev. 9(16): 2034-2041.
1/Jun/2007
Refer to NCBI
Refer to GenBank
Refer to Swiss-Prot
19452503
Western Blot
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154) at 1:60 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite. | Western blot analysis of Mouse brain cell lysates showing detection of Phosphothreonine protein using Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154). Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154) at 1:1000. Left: Treated with Vanadium, Right: Non-treated.. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154) at 1:60 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite. Rabbit Anti-Phosphothreonine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-154) | Rabbit Anti-Phosphothreonine Antibody used in Western blot (WB) on Mouse brain cell lysates (SPC-154) | Rabbit Anti-Phosphothreonine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-154)
Non-hazardous
Canada
1.4
0.4
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
