Immunogen
Human p70 S6K c-terminal peptide –KLH conjugates
Antibody's target
Human p70 S6K
Antibody's full description
Rabbit Anti-Human p70 S6K Polyclonal
400 µl
SPC-146F-ALP
274€
Human p70 S6K c-terminal peptide –KLH conjugates
Human p70 S6K
Rabbit Anti-Human p70 S6K Polyclonal
Cell Signaling, Post-translational modifications, Phosphorylation
Polyclonal Antibodies
p70 S6 kinase beta (phospho T229) Antibody, p70-beta Antibody, p70S6Kb Antibody, S6K beta Antibody, S6K2 Antibody, SRK Antibody, STK14B Antibody
WB, ICC/IF, IP, ELISA
Rabbit
Human, Mouse, Rat, Bovine
WB (1:1000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Protein A Purified
TBS, 50% glycerol, 0.09% sodium azide
0.25 mg/ml
Detects ~70kDa.
-20°C
Blue Ice or 4°C
0.25 mg/ml was sufficient for detection of SPC-146 in lysates prepared from mouse muscle and mouse brain.
Cytoplasm, Nucleus, Cell Junction, Mitochondrion, Mitochondrion Outer Membrane
See included datasheet or contact our support service.
Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/ dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5’ transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70% amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and C-terminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.
1. Gout, I. et al. (1998). J Biol Chem. 273 (46):30061-30064. 2. Val’Ovka, TI. et al. (2000). Ukr Biokhim Zh. 72(3):31-7. 3. Kawasome, H. et al. (1998) Proc. Natl. Acad.Sci. 95 4. Montagne, J. et al. 1999) Science 285 5. Shima, H. et al. (1998) EMBO J 17: 6649-6659. 6. Lee-Fruman, K. et al. (1999) Oncogene 18: 5108- 5114.
1/Jun/2007
NP_003943.2
6199
Q9UBS0
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Perinuclear region of cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p70 S6K Antibody. (C) Composite. | Western blot analysis of Mouse brain cell lysates showing detection of p70 S6K protein using Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146). Primary Antibody: Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146) at 1:1000. Anti-p70 S6K Antibody on right, IP negative control on the left. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146) at 1:50 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Perinuclear region of cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p70 S6K Antibody. (C) Composite. Rabbit Anti-p70 S6K Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-146) | Rabbit Anti-p70 S6K Antibody used in Western blot (WB) on Mouse brain cell lysates (SPC-146) | Rabbit Anti-p70 S6K Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-146)
Non-hazardous
Canada
1.4
0.4
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
