Immunogen
A 19 residue synthetic peptide based on dog calnexin and the peptide coupled to KLH
Antibody's target
Human Calnexin
Antibody's full description
Rabbit Anti-Human Calnexin-NT Polyclonal
200 µl
SPC-127B-ALP
462€
A 19 residue synthetic peptide based on dog calnexin and the peptide coupled to KLH
Human Calnexin
Rabbit Anti-Human Calnexin-NT Polyclonal
Cell Signaling, Organelle Markers
Polyclonal Antibodies
Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody
WB, IHC, ICC/IF, IP
Rabbit
Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig (Cavia porcellus), Hamster, Pig, Monkey, Rabbit, Sheep, African clawed frog (Xenopus laevis)
WB (1:5000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user.
Rabbit Antiserum
Rabbit Antiserum
See included datasheet or contact our support service
Detects the N-terminal domain of Calnexin ~90kDa.
4°C
Blue Ice or 4°C
A 1:5000 dilution of SPC-127 was sufficient for detection of Calnexin in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Melanosome
See included datasheet or contact our support service.
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33:3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49):40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6. 8. Raggo C., et al. (2002) Mol Cell Biol. 22: 5639-5649. 9. Rubio M.E., and Wenthold R.J. (1999) J Neurochem. 73: 942-948.
1/May/2008
NP_001003232.1
403908
P24643
22180459
Immunocytochemistry/Immunofluorescence
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-127). Tissue: HeLa cells. Species: Human. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-127) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green). | Western blot analysis of Human HeLa cell lysates showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-127). Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-127) at 1:1000. | Immunohistochemistry analysis using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-127). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-127) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Upper layer staining and few basal cell cytoplasmic staining. | Western blot analysis of Rat tissue mix showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-127). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-127) at 1:5000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunohistochemistry analysis using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-127). Tissue: colon colitis. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-127) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Localization: Inflammatory cells. Rabbit Anti-Calnexin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa cells (SPC-127) | Rabbit Anti-Calnexin Antibody used in Western blot (WB) on Human HeLa cell lysates (SPC-127) | Rabbit Anti-Calnexin Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-127) | Rabbit Anti-Calnexin Antibody used in Western blot (WB) on Rat tissue mix (SPC-127) | Rabbit Anti-Calnexin Antibody used in Immunohistochemistry (IHC) on Mouse colon colitis (SPC-127)
Non-hazardous
Canada
1.4
0.2
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
