Immunogen
Peptide corresponding to AA 227-236 of human EC SOD
Antibody's target
Human SOD3
Antibody's full description
Rabbit Anti-Human SOD (EC) Polyclonal
100 µg
SPC-124D-ALP
419€
Peptide corresponding to AA 227-236 of human EC SOD
Human SOD3
Rabbit Anti-Human SOD (EC) Polyclonal
Cancer, Oxidative Stress, Neuroscience, Neurodegeneration, ALS Disease
Polyclonal Antibodies
EC SOD antibody, EC-SOD antibody, Extracellular superoxide dismutase [Cu Zn] antibody, Extracellular superoxide dismutase [Cu-Zn] antibody, Extracellular superoxide dismutase antibody, Extracellular superoxide dismutase precursor antibody, MGC20077 antibody, SOD 3 antibody, SOD3 antibody, SODE_HUMAN antibody, Superoxide dismutase 3 extracellular antibody
WB, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects extracellular SOD ~35kDa.
-20°C
Blue Ice or 4°C
1 µg/ml of SPC-124 was sufficient for detection of ECSOD in 20 µg of Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Extracellular Space
See included datasheet or contact our support service.
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).
1. Adachi T., et al. (1992) Clin Chim Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. FurukawaY., and O’Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6. 4. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988) Free Radical Biol. Med. 5: 377-385. 6. Wispe J.R., et al. (1989) BBA. 994: 30-36. 7. Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491
9/May/2013
P08294
6649
P08294
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (EC) Polyclonal Antibody (SPC-124). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody (SPC-124) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Golgi lumen. Exosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (EC) Antibody. (C) Composite. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (EC) Polyclonal Antibody (SPC-124). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody (SPC-124) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Golgi lumen. Exosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (EC) Antibody. (C) Composite. | Western blot analysis of Human Cervical Cancer cell lysates (HeLa) showing detection of ~35 kDa SOD (EC) protein using Rabbit Anti-SOD (EC) Polyclonal Antibody (SPC-124). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical Cancer cell lysates (HeLa). Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody (SPC-124) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35 kDa. Other Band(s): 50 kDa. Rabbit Anti-SOD (EC) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-124) | Rabbit Anti-SOD (EC) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-124) | Rabbit Anti-SOD (EC) Antibody used in Western blot (WB) on Cervical Cancer cell lysates (HeLa) (SPC-124)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
