Immunogen
Native bovine Ubiquitin, conjugated to KLH
Antibody's target
Bovine Ubiquitin
Antibody's full description
Rabbit Anti-Bovine Ubiquitin Polyclonal
200 µl
SPC-119B-ALP
433€
Native bovine Ubiquitin, conjugated to KLH
Bovine Ubiquitin
Rabbit Anti-Bovine Ubiquitin Polyclonal
Cell Signaling, Post-Translational Modifications, Ubiquitination
Polyclonal Antibodies
Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
WB, IHC, ICC/IF, IP
Rabbit
Human, Mouse, Rat, Bovine, Monkey, Hamster, Rabbit, Guinea Pig (Cavia porcellus), Pig, Dog, Sheep, Chicken, African clawed frog (Xenopus laevis), Yeast, Fruit Fly (Drosophila melanogaster), Fish, Rainbow Trout (Oncorhynchus mykiss)
WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~10kDa. It also recognizes ubiquinated proteins.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-119 was sufficient for detection of free ubiquitin in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
Cell Membrane, Cytoplasm, Nucleus
See included datasheet or contact our support service.
Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (6).
1. Wilkinson K.D. (1995) Annu. Rev. Nutr. 15:161-189. 2. Smalle J., Vierstra R.D. (2004) Anu Rev Plant Biol. 55: 555-590. 3. Bonifacino J.S., et al. (1998) Annu Rev Cell Dev Biol. 14: 19-57. 4. Boston Biochem: "Ubiquitin Proteasome Pathway Overview” 5. Yang M., et al. (1998) J Exp Med. 187: 1835-1846. 6. Chen Z.J., et al. (1996) Cell 84: 853-862.
1/Oct/2008
NP_776558.1
281370
P0CG53
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Human HEK93 lysates showing detection of Ubiquitin protein using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:1000. | Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm. | Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite. Rabbit Anti-Ubiquitin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-119) | Rabbit Anti-Ubiquitin Antibody used in Western blot (WB) on Human HEK93 lysates (SPC-119) | Rabbit Anti-Ubiquitin Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-119) | Rabbit Anti-Ubiquitin Antibody used in Immunohistochemistry (IHC) on Human colon carcinoma (SPC-119) | Rabbit Anti-Ubiquitin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-119)
Non-hazardous
Canada
1.4
0.2
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
