Immunogen
Rat PDI synthetic peptide conjugated to KLH
Antibody's target
Rat PDI
Antibody's full description
Rabbit Anti-Rat PDI Polyclonal
100 µl
SPC-114D-ALP
394€
Rat PDI synthetic peptide conjugated to KLH
Rat PDI
Rabbit Anti-Rat PDI Polyclonal
Cell Signaling, Trafficking, Chaperones, Organelle Markers
Polyclonal Antibodies
PDA2 Antibody, PDI Antibody, PDIA2 Antibody, PDIP Antibody, pancreatic protein disulfide isomerase Antibody
WB, IHC, ICC/IF, IP
Rabbit
Human, Mouse, Rat, Bovine, Dog, Hamster, Monkey, Guinea Pig (Cavia porcellus), Sheep, Pig, African clawed frog (Xenopus laevis), Mollusk, Mussel (Perna viridis)
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~58kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-114 was sufficient for detection of PDI in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Endoplasmic Reticulum, Endoplasmic Reticulum Lumen
See included datasheet or contact our support service.
The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).
1. NA K.S. et al. (2007) Mol Cells. 24(2): 261-7. 2. Mayer M., Kies U., Kammermeier R., and Buchner J. (2000) J Biol Chem. 275(38): 29421-5. 3. Delom F., Mallet B., Carayon P., and Lejeune P.J. (2001) J Biol Chem 276(24): 21337-42. 4. Schultz-Norton J.R., McDonald W.H., Yates J.R. and Nardulli A.M. (2006) Mol Endocrinol 20(9): 1982-95. 5. Turano C., Coppari S. Altieri F. and Ferraro (2002) J Cell Physiol 193: 154-163. 6. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819. 7. Yoshimori T., et al. (1990) J Biol Chem. 265(26): 15984-90.
1/Jun/2008
NP_001099245.2
287164
P04785
25678563|25678563|21684241
Western Blot|Western Blot|Western Blot
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-PDI Antibody. (C) Composite. | Western blot analysis of Rat tissue mix showing detection of PDI protein using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Load: 15 µg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PDI Antibody. (C) Composite. Rabbit Anti-PDI Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-114) | Rabbit Anti-PDI Antibody used in Western blot (WB) on Rat tissue mix (SPC-114) | Rabbit Anti-PDI Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-114)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Oryctolagus cuniculus,Rattus norvegicus
