Immunogen
Human heme-oxygenase (HO-1) synthetic multiple antigenic peptide
Antibody's target
Human HO-1
Antibody's full description
Rabbit Anti-Human HO-1 Polyclonal
100 µg
SPC-112D-ALP
340€
Human heme-oxygenase (HO-1) synthetic multiple antigenic peptide
Human HO-1
Rabbit Anti-Human HO-1 Polyclonal
Cancer, Oxidative Stress
Polyclonal Antibodies
Heme oxygenase 1 Antibody, Hemox Antibody, HMOX1 Antibody, HO1 Antibody, HO 1 Antibody, HSP32 Antibody
WB, ICC/IF, IHC
Rabbit
Human, Mouse, Rat, Dog
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Protein A Purified
PBS pH7.4, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~32kDa.
-20°C
Blue Ice or 4°C
1 µg/ml of SPC-112 was sufficient for detection of HO-1 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Endoplasmic Reticulum, Microsome
See included datasheet or contact our support service.
Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.
1/Nov/2008
NP_002124.1
3162
P09601
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. Heat Shocked at 42°C for 1h. | Western blot analysis of Human Cell line lysates showing detection of HO-1 protein using Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112). Load: 15 µg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite. Rabbit Anti-HO-1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-112) | Rabbit Anti-HO-1 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-112) | Rabbit Anti-HO-1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-112) | Rabbit Anti-HO-1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-112)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
