Immunogen
Amino acids 650-670 of human HSP73
Antibody's target
Human HSC70
Antibody's full description
Rabbit Anti-Human HSC70 (HSP73) Polyclonal
100 µl
SPC-102D-ALP
360€
Amino acids 650-670 of human HSP73
Human HSC70
Rabbit Anti-Human HSC70 (HSP73) Polyclonal
Cancer, Heat Shock
Polyclonal Antibodies
HSC54 Antibody, HSC71 Antibody, HSC73 Antibody, HSP71 Antibody, HSP73 Antibody, HSPA10 Antibody, HSPA8 Antibody, LAP1 Antibody, NIP71 Antibody
WB, IHC, ICC/IF, IP, Bl
Rabbit
Human, Mouse, Rat, Hamster
WB (1:2000), IHC (1:100), ICC/IF (1:250); optimal dilutions for assays should be determined by the user.
Rabbit Antiserum
Rabbit Antiserum
See included datasheet or contact our support service
Detects 73kDa. Does not cross-react with HSP70.
4°C
Blue Ice or 4°C
0.5 µg/ml of SPC-102 was sufficient for detection of HSC70 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Melanosome
See included datasheet or contact our support service.
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g. heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
1. Brown C.L. et al. (1993) J.Cell Biol., 120 (5): 1101-1112. 2. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989), Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA 89: 7290-7294. 6. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.
1/Jun/2007
NP_006588.1
3312
P11142
24361285|24447452|24447452|24056538|23060796|20018937|19209902
Immunoprecipitation|Immunohistochemistry|Western Blot |Immunocytochemistry/Immunofluorescence|Western Blot|Western Blot|Western Blot
Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm. | Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. | Western blot analysis of Human Cell line lysates showing detection of HSC70 protein using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:50000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Rabbit Anti-HSC70 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-102) | Rabbit Anti-HSC70 Antibody used in Immunohistochemistry (IHC) on Human colon carcinoma (SPC-102) | Rabbit Anti-HSC70 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-102) | Rabbit Anti-HSC70 Antibody used in Immunohistochemistry (IHC) on Mouse Inflamed colon (SPC-102)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
