Category
Proteins
Antibody Subtype
Conjugated Proteins
Area of research
Miscellaneous
Proteins
Conjugated Proteins
Miscellaneous
N/A
Alkaline Phosphatase
Synthetic
Biotin reagent (Alk Phos) was purified by ion exchange chromatography followed by dialysis.
1.0 mg/ml
0.05M TRIS-HCl, pH 8.0, with 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
Store at 4 deg C. DO NOT FREEZE.
Blue Ice
ELISA; IHC; WB
ELISA: 1:8,000-1:32,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Biotinylated
