Primary Antibody
Conjugated Polyclonal Antibodies
Proteases, Inhibitors, & Enzymes
Alkaline phosphatase antibody (HRP) was raised in rabbit using alkaline phosphatase isolated from E.coli ..
Rabbit
E Coli alkaline phosphotase
Should be determined by the end user
HRP
Alkaline Phosphatase antibody (E. Coli) (HRP) was purified by ion exchange chromatography followed by dialysis.
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3. I mMunoglobulin and protease free.
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Blue Ice
ELISA; WB
ELISA: 1:4,000-1:20,000, WB: 1:500-1:2,000
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
HRP Conjugated
anticorps