Alernative name
Rabbit Anti- DYKDDDDK Tag Polyclonal
Target
DYKDDDDK Tag
Conjugate
Alkaline Phosphatase
100 µg
SPC-720D-ALP
485€
Rabbit Anti- DYKDDDDK Tag Polyclonal
DYKDDDDK Tag
Alkaline Phosphatase
Tags and Cell Markers , Epitope Tags
Antibodies
Polyclonal
Synthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH).
NA
NA
NA
WB
Rabbit
Species Independent
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
1 mg/ml
Binds to DYKDDDDK (FLAG tag) conjugated proteins. Recongizes the same epitope as Sigma's Anti-FLAG® antibodies.
-20°C
Blue Ice or 4°C
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
Flag
anticorps