Recombinant Mouse PTPN6 Protein (aa 207-597, His & GST tag)

Size

20µg

Catalog No

PKSM040450-20µg

Price

356€

Synonym

70Z-SHP;hcp;Hcph;me;Ptp1C;PTPTY-42;SH-PTP1;SHP-1

Activity

Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988998 phosphopeptide substrate, R&D Systems, Catalog # ES006.The specific activity is > 4 μmoles/min/μg.

Sequence

Ala207-Lys597

Recombinant Mouse PTPN6 Protein (aa 207-597, His & GST tag)

Fusion tag

N-His & GST

Accession

P29351-2

Expressed Host

Baculovirus-Insect Cells

Recombinant Mouse PTPN6 Protein (aa 207-597, His & GST tag)

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Purity

> 85 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Recombinant Mouse PTPN6 Protein (aa 207-597, His & GST tag)

Stability and Storage

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Mol Mass

72.7 kDa

AP Mol Mass

63 kDa

Recombinant Mouse PTPN6 Protein (aa 207-597, His & GST tag)

Formulation

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0, 10% glycerol

Background

PTPN6 is an enzyme which belongs to the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of PTPN6 contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of PTPN6 with its substrates. PTPN6 is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. It has been shown that PTPN6 interacts with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling.

Conjugation

histidine

Recombinant Mouse PTPN6 Protein (aa 207-597, His & GST tag)

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus

Source

Recombinants or rec. proteins

Recombinant Mouse PTPN6 Protein (aa 207-597, His & GST tag)

Group

recombinants

Recombinant Mouse PTPN6 Protein (aa 207-597, His & GST tag)